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PDBsum entry 1o1g

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Contractile protein PDB id
1o1g
Jmol
Contents
Protein chains
(+ 0 more) 840 a.a.
(+ 0 more) 145 a.a.
(+ 0 more) 147 a.a.
(+ 8 more) 372 a.a.

References listed in PDB file
Key reference
Title Molecular modeling of averaged rigor crossbridges from tomograms of insect flight muscle.
Authors L.F.Chen, H.Winkler, M.K.Reedy, M.C.Reedy, K.A.Taylor.
Ref. J Struct Biol, 2002, 138, 92-104. [DOI no: 10.1016/S1047-8477(02)00013-8]
PubMed id 12160705
Abstract
Electron tomography, correspondence analysis, molecular model building, and real-space refinement provide detailed 3-D structures for in situ myosin crossbridges in the nucleotide-free state (rigor), thought to represent the end of the power stroke. Unaveraged tomograms from a 25-nm longitudinal section of insect flight muscle preserved native structural variation. Recurring crossbridge motifs that repeat every 38.7 nm along the actin filament were extracted from the tomogram and classified by correspondence analysis into 25 class averages, which improved the signal to noise ratio. Models based on the atomic structures of actin and of myosin subfragment 1 were rebuilt to fit 11 class averages. A real-space refinement procedure was applied to quantitatively fit the reconstructions and to minimize steric clashes between domains introduced during the fitting. These combined procedures show that no single myosin head structure can fit all the in situ crossbridges. The validity of the approach is supported by agreement of these atomic models with fluorescent probe data from vertebrate muscle as well as with data from regulatory light chain crosslinking between heads of smooth muscle heavy meromyosin when bound to actin.
Secondary reference #1
Title Real space refinement of acto-Myosin structures from sectioned muscle.
Authors L.F.Chen, E.Blanc, M.S.Chapman, K.A.Taylor.
Ref. J Struct Biol, 2001, 133, 221-232. [DOI no: 10.1006/jsbi.2000.4321]
PubMed id 11472093
Abstract
Secondary reference #2
Title Multivariate statistical analysis of three-Dimensional cross-Bridge motifs in insect flight muscle
Authors H.Winkler, K.A.Taylor.
Ref. ultramicroscopy, 1999, 77, 141.
Secondary reference #3
Title The use of electron tomography for structural analysis of disordered protein arrays.
Authors K.A.Taylor, J.Tang, Y.Cheng, H.Winkler.
Ref. J Struct Biol, 1997, 120, 372-386. [DOI no: 10.1006/jsbi.1997.3932]
PubMed id 9441940
Full text Abstract
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