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PDBsum entry 1o18
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Contractile protein
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PDB id
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1o18
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Contents |
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(+ 0 more)
840 a.a.
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145 a.a.
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147 a.a.
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(+ 8 more)
372 a.a.
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References listed in PDB file
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Key reference
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Title
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Molecular modeling of averaged rigor crossbridges from tomograms of insect flight muscle.
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Authors
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L.F.Chen,
H.Winkler,
M.K.Reedy,
M.C.Reedy,
K.A.Taylor.
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Ref.
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J Struct Biol, 2002,
138,
92-104.
[DOI no: ]
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PubMed id
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Abstract
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Electron tomography, correspondence analysis, molecular model building, and
real-space refinement provide detailed 3-D structures for in situ myosin
crossbridges in the nucleotide-free state (rigor), thought to represent the end
of the power stroke. Unaveraged tomograms from a 25-nm longitudinal section of
insect flight muscle preserved native structural variation. Recurring
crossbridge motifs that repeat every 38.7 nm along the actin filament were
extracted from the tomogram and classified by correspondence analysis into 25
class averages, which improved the signal to noise ratio. Models based on the
atomic structures of actin and of myosin subfragment 1 were rebuilt to fit 11
class averages. A real-space refinement procedure was applied to quantitatively
fit the reconstructions and to minimize steric clashes between domains
introduced during the fitting. These combined procedures show that no single
myosin head structure can fit all the in situ crossbridges. The validity of the
approach is supported by agreement of these atomic models with fluorescent probe
data from vertebrate muscle as well as with data from regulatory light chain
crosslinking between heads of smooth muscle heavy meromyosin when bound to actin.
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Secondary reference #1
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Title
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Real space refinement of acto-Myosin structures from sectioned muscle.
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Authors
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L.F.Chen,
E.Blanc,
M.S.Chapman,
K.A.Taylor.
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Ref.
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J Struct Biol, 2001,
133,
221-232.
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PubMed id
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Secondary reference #2
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Title
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Multivariate statistical analysis of three-Dimensional cross-Bridge motifs in insect flight muscle
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Authors
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H.Winkler,
K.A.Taylor.
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Ref.
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ultramicroscopy, 1999,
77,
141.
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Secondary reference #3
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Title
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The use of electron tomography for structural analysis of disordered protein arrays.
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Authors
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K.A.Taylor,
J.Tang,
Y.Cheng,
H.Winkler.
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Ref.
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J Struct Biol, 1997,
120,
372-386.
[DOI no: ]
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PubMed id
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