PDBsum entry 1o04

Go to PDB code: 
Top Page protein ligands metals Protein-protein interface(s) links
Oxidoreductase PDB id
Protein chain
(+ 2 more) 494 a.a. *
NAD ×8
GAI ×18
EDO ×32
_NA ×8
_MG ×8
Waters ×5025
* Residue conservation analysis

References listed in PDB file
Key reference
Title Coenzyme isomerization is integral to catalysis in aldehyde dehydrogenase.
Authors S.J.Perez-Miller, T.D.Hurley.
Ref. Biochemistry, 2003, 42, 7100-7109. [DOI no: 10.1021/bi034182w]
PubMed id 12795606
Crystal structures of many enzymes in the aldehyde dehydrogenase superfamily determined in the presence of bound NAD(P)(+) have exhibited conformational flexibility for the nicotinamide half of the cofactor. This has been hypothesized to be important in catalysis because one conformation would block the second half of the reaction, but no firm evidence has been put forth which shows whether the oxidized and reduced cofactors preferentially occupy the two observed conformations. We present here two structures of the wild type and two structures of a Cys302Ser mutant of human mitochondrial aldehyde dehydrogenase in binary complexes with NAD(+) and NADH. These structures, including the Cys302Ser mutant in complex with NAD(+) at 1.4 A resolution and the wild-type enzyme in complex with NADH at 1.9 A resolution, provide strong evidence that bound NAD(+) prefers an extended conformation ideal for hydride transfer and bound NADH prefers a contracted conformation ideal for acyl-enzyme hydrolysis. Unique interactions between the cofactor and the Rossmann fold make isomerization possible while allowing the remainder of the active site complex to remain intact. In addition, these structures clarify the role of magnesium in activating the human class 2 enzyme. Our data suggest that the presence of magnesium may lead to selection of particular conformations and speed isomerization of the reduced cofactor following hydride transfer.
Go to PROCHECK summary