PDBsum entry 1nya

Metal binding protein

PDB id: 1nya

Contents

Protein chain

176 a.a. *

Metals

_CA ×3

* Residue conservation analysis

References listed in PDB file

Key reference

Title

Nmr solution structure of calerythrin, An ef-Hand calcium-Binding protein from saccharopolyspora erythraea.

Authors

H.Tossavainen, P.Permi, A.Annila, I.Kilpeläinen, T.Drakenberg.

Ref.

Eur J Biochem, 2003, 270, 2505-2512. [DOI no: 10.1046/j.1432-1033.2003.03623.x]

PubMed id

12755706

Abstract

The structure of calerythrin, a prokaryotic 20 kDa calcium-binding protein has been determined by solution NMR spectroscopy. Distance, dihedral angle, J coupling, secondary chemical shift, residual dipolar coupling and radius of gyration restraints reveal four EF-hand motifs arranged in a compact globular structure. A tight turn in the middle of the amino acid sequence brings the two halves, each comprising a pair of EF-hands, close together. The structural similarity between calerythrin and the eukaryotic sarcoplasmic calcium-binding proteins is notable.

Figure 4.
Fig. 4. Ribbon representation of the refined lowest-energy structure. EF-hands are represented by different colours, EF-1 yellow, EF-2 red, EF-3 green and EF-4 blue. The short -sheet is represented by arrows. The kink between the halves can be seen between helix D (red) and helix E (green). Calcium ions are represented as orange spheres.

Figure 5.
Fig. 5. A stereo view of calerythrin superimposed with N-SCP. The colour code is the same as in Fig. 4 Go-.

The above figures are reprinted by permission from the Federation of European Biochemical Societies: Eur J Biochem (2003, 270, 2505-2512) copyright 2003.

Secondary reference #1

Title

Nmr assignments, Secondary structure, And global fold of calerythrin, An ef-Hand calcium-Binding protein from saccharopolyspora erythraea.

Authors

H.Aitio, A.Annila, S.Heikkinen, E.Thulin, T.Drakenberg, I.Kilpeläinen.

Ref.

Protein Sci, 1999, 8, 2580-2588. [DOI no: 10.1110/ps.8.12.2580]

PubMed id

10631973