PDBsum entry 1no8

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protein links
RNA binding protein PDB id
Protein chain
78 a.a. *
* Residue conservation analysis
PDB id:
Name: RNA binding protein
Title: Solution structure of the nuclear factor aly rbd domain
Structure: Aly. Chain: a. Engineered: yes
Source: Mus musculus. House mouse. Organism_taxid: 10090. Gene: aly. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
NMR struc: 32 models
Authors: G.C.Perez-Alvarado,M.Martinez-Yamout,M.M.Allen,R.Grosschedl, H.J.Dyson,P.E.Wright
Key ref:
G.C.Pérez-Alvarado et al. (2003). Structure of the nuclear factor ALY: insights into post-transcriptional regulatory and mRNA nuclear export processes. Biochemistry, 42, 7348-7357. PubMed id: 12809490 DOI: 10.1021/bi034062o
15-Jan-03     Release date:   12-Aug-03    
Go to PROCHECK summary

Protein chain
Pfam   ArchSchema ?
O08583  (THOC4_MOUSE) -  THO complex subunit 4
255 a.a.
78 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Gene Ontology (GO) functional annotation 
  GO annot!
  Biochemical function     nucleotide binding     2 terms  


DOI no: 10.1021/bi034062o Biochemistry 42:7348-7357 (2003)
PubMed id: 12809490  
Structure of the nuclear factor ALY: insights into post-transcriptional regulatory and mRNA nuclear export processes.
G.C.Pérez-Alvarado, M.Martínez-Yamout, M.M.Allen, R.Grosschedl, H.J.Dyson, P.E.Wright.
ALY is a ubiquitously expressed nuclear protein which interacts with proteins such as TAP that are involved in export of mRNA from the nucleus to the cytoplasm, as well as with proteins that bind the T cell receptor alpha gene enhancer. ALY has also been shown to bind mRNA and to co-localize in the nucleus with components of a multiprotein postsplicing complex that is deposited 20-24 nucleotides upstream of exon-exon junctions. ALY has a conserved RNA binding domain (RBD) flanked by Gly-Arg rich N-terminal and C-terminal sequences. We determined the solution structure of the RBD homology region in ALY by nuclear magnetic resonance methods. The RBD motif in ALY has a characteristic beta(1)alpha(1)beta(2)-beta(3)alpha(2)beta(4) fold, consisting of a beta sheet composed of four antiparallel beta strands and two alpha helices that pack on one face of the beta sheet. As in other RBD structures, the beta sheet has an exposed face with hydrophobic and charged residues that could modulate interactions with other molecules. The loop that connects beta strands 2 and 3 is in intermediate motion in the NMR time scale, which is also characteristic of other RBDs. This loop presents side chains close to the exposed surface of the beta sheet and is a primary candidate site for intermolecular interactions. The structure of the conserved RBD of ALY provides insight into the nature of interactions involving this multifunctional protein.

Literature references that cite this PDB file's key reference

  PubMed id Reference
21253573 R.B.Tunnicliffe, G.M.Hautbergue, P.Kalra, B.R.Jackson, A.Whitehouse, S.A.Wilson, and A.P.Golovanov (2011).
Structural basis for the recognition of cellular mRNA export factor REF by herpes viral proteins HSV-1 ICP27 and HVS ORF57.
  PLoS Pathog, 7, e1001244.
PDB code: 2kt5
18368466 J.Siltberg-Liberles, and A.Martinez (2009).
Searching distant homologs of the regulatory ACT domain in phenylalanine hydroxylase.
  Amino Acids, 36, 235-249.  
18417565 L.J.Marshall, A.C.Moore, M.Ohki, I.Kitabayashi, D.Patterson, and D.A.Ornelles (2008).
RUNX1 permits E4orf6-directed nuclear localization of the adenovirus E1B-55K protein and associates with centers of viral DNA and RNA synthesis.
  J Virol, 82, 6395-6408.  
17506639 A.Cook, F.Bono, M.Jinek, and E.Conti (2007).
Structural biology of nucleocytoplasmic transport.
  Annu Rev Biochem, 76, 647-671.  
17000901 A.P.Golovanov, G.M.Hautbergue, A.M.Tintaru, L.Y.Lian, and S.A.Wilson (2006).
The solution structure of REF2-I reveals interdomain interactions and regions involved in binding mRNA export factors and RNA.
  RNA, 12, 1933-1948.
PDB code: 2f3j
16738786 A.P.Golovanov, G.M.Hautbergue, S.A.Wilson, and L.Y.Lian (2006).
Assignment of 1H, 13C, and 15N resonances for the REF2-I mRNA export factor.
  J Biomol NMR, 36, 41.  
16418270 R.C.Wilson, C.J.Bohlen, M.P.Foster, and C.E.Bell (2006).
Structure of Pfu Pop5, an archaeal RNase P protein.
  Proc Natl Acad Sci U S A, 103, 873-878.
PDB code: 2av5
15853797 C.Maris, C.Dominguez, and F.H.Allain (2005).
The RNA recognition motif, a plastic RNA-binding platform to regulate post-transcriptional gene expression.
  FEBS J, 272, 2118-2131.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.