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PDBsum entry 1nik
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Transcription
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PDB id
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1nik
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Contents |
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1388 a.a.
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1097 a.a.
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266 a.a.
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153 a.a.*
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214 a.a.
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84 a.a.
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170 a.a.*
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133 a.a.
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119 a.a.
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65 a.a.
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114 a.a.
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46 a.a.
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* C-alpha coords only
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References listed in PDB file
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Key reference
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Title
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Complete, 12-Subunit RNA polymerase ii at 4.1-A resolution: implications for the initiation of transcription.
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Authors
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D.A.Bushnell,
R.D.Kornberg.
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Ref.
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Proc Natl Acad Sci U S A, 2003,
100,
6969-6973.
[DOI no: ]
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PubMed id
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Abstract
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The x-ray structure of complete RNA polymerase II from Saccharomyces cerevisiae
has been determined, including a heterodimer of subunits Rpb4 and Rpb7 not
present in previous "core" polymerase II structures. The heterodimer
maintains the polymerase in the conformation of a transcribing complex, may bind
RNA as it emerges from the enzyme, and is in a position to interact with general
transcription factors and the Mediator of transcriptional regulation.
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Figure 2.
Fig. 2. Backbone model of complete, 12-subunit pol II.
Shown is a ribbon representation of the complete pol II
structure (top and back views as in refs. 10 and 11). Rpb1 is
gray, Rpb2 is bronze, Rpb4 is red, Rpb6 is green, the N-terminal
half of Rpb7 that contains the RNP domain is dark blue, the
C-terminal half of Rpb7 that contains the OB fold is light blue,
and the remaining subunits are black. The locations of the
clamp, the C-terminal repeat domain (CTD), and the previously
proposed RNA exit groove 1 (pink dashed line) are indicated. The
figure was generated with SWISS-PDB VIEWER and POV-RAY (40).
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Figure 3.
Fig. 3. Relationship of complete pol II x-ray structure to
EM structures of complete pol II (A) and Mediator-pol II complex
(B). As this complex was prepared from exponentially growing
yeast, it would have been largely deficient in Rpb4/Rpb7,
accounting for the lack of density in this region of the EM map.
The core pol II model is blue in A and yellow in B. Rpb4 is red
and Rpb7 is dark blue. The figure was generated by using O and
POV-RAY (19).
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