 |
PDBsum entry 1ngc
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Hydrolase(acting on acid anhydrides)
|
PDB id
|
|
|
|
1ngc
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
References listed in PDB file
|
|
|
Key reference
|
|
|
Title
|
|
Structural basis of the 70-Kilodalton heat shock cognate protein ATP hydrolytic activity. Ii. Structure of the active site with ADP or ATP bound to wild type and mutant atpase fragment.
|
|
|
Authors
|
|
K.M.Flaherty,
S.M.Wilbanks,
C.Deluca-Flaherty,
D.B.Mckay.
|
|
|
Ref.
|
|
J Biol Chem, 1994,
269,
12899-12907.
|
|
|
PubMed id
|
|
|
|
|
|
Abstract
|
|
|
The ATPase fragment of the bovine 70-kDa heat shock cognate protein is an
attractive construct in which to study its mechanism of ATP hydrolysis. The
three-dimensional structure suggests several residues that might participate in
the ATPase reaction. Four acidic residues (Asp-10, Glu-175, Asp-199, and
Asp-206) have been individually mutated to both the cognate amine
(asparagine/glutamine) and to serine, and the effects of the mutations on the
kinetics of the ATPase activity (Wilbanks, S. M., DeLuca-Flaherty, C., and
McKay, D. B. (1994) J. Biol. Chem. 269, 12893-12898) and the structure of the
mutant ATPase fragments have been determined, typically to approximately 2.4 A
resolution. Additionally, the structures of the wild type protein complexed with
MgADP and Pi, MgAMPPNP (5'-adenylyl-beta, gamma-imidodiphosphate) and CaAMPPNP
have been refined to 2.1, 2.4, and 2.4 A, respectively. Combined, these
structures provide models for the prehydrolysis, MgATP-bound state and the
post-hydrolysis, MgADP-bound state of the ATPase fragment. These models suggest
a pathway for the hydrolytic reaction in which 1) the gamma phosphate of bound
ATP reorients to form a beta, gamma-bidentate phosphate complex with the Mg2+
ion, allowing 2) in-line nucleophilic attack on the gamma phosphate by a H2O
molecule or OH- ion, with 3) subsequent release of inorganic phosphate.
|
|
|
Secondary reference #1
|
|
|
Title
|
|
Three-Dimensional structure of the atpase fragment of a 70k heat-Shock cognate protein.
|
|
|
Authors
|
|
K.M.Flaherty,
C.Deluca-Flaherty,
D.B.Mckay.
|
|
|
Ref.
|
|
Nature, 1990,
346,
623-628.
|
|
|
PubMed id
|
|
|
|
|
|
|
|
|
|
|
|
|
