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UniProt functional annotation for Q9H0M0 | ||
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| UniProt code: Q9H0M0. |
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| Organism: | |
Homo sapiens (Human). |
| Taxonomy: | |
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. |
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| Function: | |
E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Ubiquitinates ERBB4 isoforms JM-A CYT-1 and JM-B CYT-1, KLF2, KLF5 and TP63 and promotes their proteasomal degradation. Ubiquitinates RNF11 without targeting it for degradation. Ubiquitinates and promotes degradation of TGFBR1; the ubiquitination is enhanced by SMAD7. Ubiquitinates SMAD6 and SMAD7. Ubiquitinates and promotes degradation of SMAD2 in response to TGF-beta signaling, which requires interaction with TGIF. {ECO:0000269|PubMed:12535537, ECO:0000269|PubMed:15221015, ECO:0000269|PubMed:15359284}. |
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| Catalytic activity: | |
Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L- cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.; EC=2.3.2.26; |
| Activity regulation: | |
Activated by NDFIP1- and NDFIP2-binding. |
| Pathway: | |
Protein modification; protein ubiquitination. |
| Subunit: | |
Binds KLF2 AND HIVEP3 (By similarity). Binds SCNN1A, SCNN1B, SCNN1G, WBP1, WBP2, DRPLA and adenovirus type 2 PIII. Interacts with RNF11 (By similarity). Interacts with SPART. Interacts with ERBB4 isoforms JM-B CYT-1 and JM-A CYT-1. Interacts with SMAD1, SMAD2, SMAD3, SMAD5, SMAD6, SMAD7, TGFBR1 AND TGFBR2. Associates with the TGFBR1:TGFBR2 receptor complex in presence of SMAD7. Interacts with SKIL isoform 1. Interacts with TP63 isoform 1 and isoform 2. Interacts with STAMBP and RNF11. Interacts with NDFIP1 and NDFIP2 (Probable); this interaction activates the E3 ubiquitin-protein ligase. Interacts with TGIF. Interacts (via WW domains) with ARRDC1, ARRDC2 and ARRDC3 (PubMed:21191027). {ECO:0000250, ECO:0000269|PubMed:12450395, ECO:0000269|PubMed:15221015, ECO:0000269|PubMed:15359284, ECO:0000269|PubMed:19561640, ECO:0000269|PubMed:19580544, ECO:0000269|PubMed:21191027, ECO:0000269|PubMed:9169421, ECO:0000269|PubMed:9647693}. |
| Subunit: | |
(Microbial infection) Interacts with HTLV-1 protein Gag. {ECO:0000269|PubMed:17609263}. |
| Subunit: | |
(Microbial infection) Interacts with ebola virus protein VP40. {ECO:0000269|PubMed:28768865}. |
| Subcellular location: | |
Cytoplasm {ECO:0000250}. Cell membrane {ECO:0000250}; Peripheral membrane protein {ECO:0000250}. Nucleus {ECO:0000250}. |
| Tissue specificity: | |
Detected in heart, placenta, pancreas, kidney, liver, skeletal muscle, bone marrow, fetal brain, and at much lower levels in adult brain and lung. Isoform 1 and isoform 5 predominate in all tissues tested, except in testis and bone marrow, where isoform 5 is expressed at much higher levels than isoform 1. {ECO:0000269|PubMed:11779188, ECO:0000269|PubMed:9647693}. |
| Domain: | |
The WW domains mediate interaction with PPxY motif-containing proteins. {ECO:0000269|PubMed:21191027}. |
| Ptm: | |
Auto-ubiquitinated and ubiquitinated by RNF11. {ECO:0000269|PubMed:15359284, ECO:0000269|PubMed:18724389, ECO:0000269|PubMed:19343052}. |
Annotations taken from UniProtKB at the EBI.