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PDBsum entry 1ncy
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Calcium-binding protein
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PDB id
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1ncy
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References listed in PDB file
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Key reference
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Title
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X-Ray structures of mn, Cd and tb metal complexes of troponin c.
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Authors
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S.T.Rao,
K.A.Satyshur,
M.L.Greaser,
M.Sundaralingam.
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Ref.
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Acta Crystallogr D Biol Crystallogr, 1996,
52,
916-922.
[DOI no: ]
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PubMed id
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Note In the PDB file this reference is
annotated as "TO BE PUBLISHED".
The citation details given above were identified by an automated
search of PubMed on title and author
names, giving a
percentage match of
88%.
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Abstract
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The crystal structures of three metal complexes of troponin C (TnC) have been
determined and refined where the two occupied structural Ca(2+) sites in the C
domain have been substituted by Mn(2+), Cd(2+) and Tb(3+). The X-ray intensity
data were collected to 2.1, 1.8 and 1.8 A resolution, respectively, on the three
metal complexes, which are isomorphous with Ca-TnC. The three complexes have
r.m.s. deviations of 0.27, 0.25 and 0.35 A, respectively, for all protein atoms,
from Ca-TnC. Irrespective of the charge on the metal (+2 or +3), the occupied
sites 3 and 4 exhibit a distorted pentagonal bipyramidal coordination, like
Ca-TnC, with seven ligands, six from the 12-residue binding loop and the seventh
from a water molecule. Mn(2+) at site 4 seems to display a longer distance to
one of the carboxyl bidentate ligands representing an intermediate coordination
simulating the six-coordinate Mg(2+). The carboxyl O atoms of the bidentate
Glu12 are displaced on the side of the equatorial plane passing through the
remaining three ligands with one O atom closer to the plane (Delta of 0.11 to
0.76 A) than the other (Delta of 0.93 to 1.38 A). The two axial ligands are an
aspartic carboxyl O atom and a water molecule. The metal is displaced (0.18 to
0.56 A) towards the water facing the water channel.
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Figure 2.
Fig. 2. Stereo figure superposing the seven ligands for site 3 of Cd-TnC on a (a) regular pentagonal biprism and (b) a regular octahedron. For the
polyhedra, a metal-to-ligand distance of 2.4 A was used.
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The above figure is
reprinted
by permission from the IUCr:
Acta Crystallogr D Biol Crystallogr
(1996,
52,
916-922)
copyright 1996.
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Secondary reference #1
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Title
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Structure of chicken skeletal muscle troponin c at 1.78 a resolution.
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Authors
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K.A.Satyshur,
D.Pyzalska,
M.Greaser,
S.T.Rao,
M.Sundaralingam.
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Ref.
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Acta Crystallogr D Biol Crystallogr, 1994,
50,
40-49.
[DOI no: ]
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PubMed id
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Figure 7.
Fig. 7. Stereoviews of the Edmondson wheels of the long central
helix. (a) The Nterminal residues 7683 with three waters, (b)
middle of th helix, residues 8496, with eight waters and (c)
Cterminal residues 97105 with one water. Notice that the
hydrophobic residues line pposite faces of the long helix at the
two ends (a) and (c).
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Figure 10.
Fig. 10. Steeo diagram of the intermolecular inteaction between
Cysl01, with its side chain discretely ordered over two sites (one
shown as solid bond and the other as broken), and residues
2428 of the A helix of the molecule related by a 32 axis (open
bonds). The water molecule 281 interacts with both S r sites of
Cysl01 (2.50, 3.05/~,), the carbonyl O atom of Ala24 (3.35 A)
and water 363 (2.64 A). One site of S r (broken bond) forms a
hydrogen bond with SD of Met28 (2.97 ~,).
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The above figures are
reproduced from the cited reference
with permission from the IUCr
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Secondary reference #2
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Title
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Refined structure of chicken skeletal muscle troponin c in the two-Calcium state at 2-A resolution.
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Authors
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K.A.Satyshur,
S.T.Rao,
D.Pyzalska,
W.Drendel,
M.Greaser,
M.Sundaralingam.
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Ref.
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J Biol Chem, 1988,
263,
1628-1647.
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PubMed id
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Secondary reference #3
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Title
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Molecular structure of troponin c from chicken skeletal muscle at 3-Angstrom resolution.
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Authors
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M.Sundaralingam,
R.Bergstrom,
G.Strasburg,
S.T.Rao,
P.Roychowdhury,
M.Greaser,
B.C.Wang.
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Ref.
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Science, 1985,
227,
945-948.
[DOI no: ]
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PubMed id
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Secondary reference #4
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Title
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X-Ray diffraction studies of troponin-C crystals from rabbit and chicken skeletal muscles.
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Authors
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G.M.Strasburg,
M.L.Greaser,
M.Sundaralingam.
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Ref.
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J Biol Chem, 1980,
255,
3806-3808.
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PubMed id
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Secondary reference #5
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Title
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The amino acid sequence of troponin c from chicken skeletal muscle.
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Author
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J.M.Wilkinson.
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Ref.
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Febs Lett, 1976,
70,
254-256.
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PubMed id
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