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UniProt functional annotation for P19524 | ||
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| UniProt code: P19524. |
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| Organism: | |
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). |
| Taxonomy: | |
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces. |
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| Function: | |
Myosin heavy chain that is required for the cell cycle- regulated transport of various organelles and proteins for their segregation. Functions by binding with its tail domain to receptor proteins on organelles and exerting force with its N-terminal motor domain against actin filaments, thereby transporting its cargo along polarized actin cables. Essential for the delivery of secretory vesicles to sites of active growth during bud emergence and cytokinesis. Required for segregation and inheritance of peroxisomes, late Golgi compartments, mitochondria and the vacuole to the daughter cell during cell division. Also required for correct alignment of the spindle during mitosis. {ECO:0000269|PubMed:10931864, ECO:0000269|PubMed:11285273, ECO:0000269|PubMed:11381095, ECO:0000269|PubMed:11733545, ECO:0000269|PubMed:11781333, ECO:0000269|PubMed:12391144, ECO:0000269|PubMed:12743102, ECO:0000269|PubMed:21248204}. |
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| Subunit: | |
Homodimer. Interacts with calmodulin (CMD1) and the myosin light chain MLC1 through its IQ repeats. Binds to the membrane receptors SEC4 and VAC17 to transport secretory vesicles and the vacuole, respectively. Binds to KAR9, which transports BIM1-coated cytoplasmic microtubules that are attached to the spindle pole body into the emerging bud, thereby correctly orienting the mitotic spindle. Interacts with YPT11 and MMR1 to accelerate mitochondrial distribution to the bud. Interacts with SHE4 and localizes it to the bud tip. Interacts with RHO3 and SMY1, putative regulators of MYO2 function. Interacts with SRO7. {ECO:0000269|PubMed:10207081, ECO:0000269|PubMed:10679024, ECO:0000269|PubMed:10984058, ECO:0000269|PubMed:12351846, ECO:0000269|PubMed:12391144, ECO:0000269|PubMed:12456647, ECO:0000269|PubMed:12554638, ECO:0000269|PubMed:12642614, ECO:0000269|PubMed:12725728, ECO:0000269|PubMed:15201867, ECO:0000269|PubMed:15964280, ECO:0000269|PubMed:21248204, ECO:0000269|PubMed:8294515, ECO:0000269|PubMed:9700160}. |
| Subcellular location: | |
Bud neck {ECO:0000269|PubMed:8188749}. Bud tip {ECO:0000269|PubMed:8188749}. Note=Concentrates to sites of polarized growth, namely to the bud tip during S and G2 phases of the cell cycle and to the bud neck during cytokinesis. |
| Domain: | |
The myosin motor domain binds to actin. |
| Domain: | |
The IQ domains provide the interaction surface for the myosin light chain MLC1. |
| Domain: | |
The coiled-coiled domain is necessary for dimerization. |
| Domain: | |
The tail domain is a globular cargo-binding domain involved in vectorial vesicle transport. |
| Miscellaneous: | |
Present with 4339 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}. |
| Miscellaneous: | |
Moves with an average velocity of 3 um/s along actin cables. |
| Similarity: | |
Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.