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PDBsum entry 1mrt
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Metallothionein
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PDB id
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1mrt
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References listed in PDB file
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Key reference
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Title
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Conformation of [cd7]-Metallothionein-2 from rat liver in aqueous solution determined by nuclear magnetic resonance spectroscopy.
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Authors
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P.Schultze,
E.Wörgötter,
W.Braun,
G.Wagner,
M.Vasák,
J.H.Kägi,
K.Wüthrich.
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Ref.
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J Mol Biol, 1988,
203,
251-268.
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PubMed id
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Abstract
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The three-dimensional structure of [Cd7]-metallothionein-2 from rat liver was
determined in aqueous solution, using nuclear magnetic resonance spectrometry
and distance geometry calculations. The experimental data provided proton-proton
distance constraints from measurements of nuclear Overhauser effects,
constraints on the geometry of the metal-cysteine clusters determined by
heteronuclear correlation spectroscopy, and dihedral angle constraints derived
from both coupling constants and nuclear Overhauser effects. The structure
calculations were performed with the program DISMAN. As in previous studies with
rabbit liver metallothionein-2a, the structure calculations were performed
separately for the alpha and beta-domains containing the 4 and 3-metal clusters,
respectively, since no interdomain constraints were found. For both domains, the
global polypeptide fold, the location of polypeptide secondary structure
elements, the architecture of the metal-sulfur cluster and the local chirality
of the metal co-ordination are very similar to the solution structure of rabbit
metallothionein-2a, but show considerable difference relative to the crystal
structure of rat metallothionein-2.
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Secondary reference #1
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Title
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Three-Dimensional structure of rabbit liver [cd7]metallothionein-2a in aqueous solution determined by nuclear magnetic resonance.
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Authors
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A.Arseniev,
P.Schultze,
E.Wörgötter,
W.Braun,
G.Wagner,
M.Vasák,
J.H.Kägi,
K.Wüthrich.
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Ref.
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J Mol Biol, 1988,
201,
637-657.
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PubMed id
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Secondary reference #2
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Title
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Sequence-Specific 1h-Nmr assignments in rat-Liver metallothionein-2.
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Authors
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E.Wörgötter,
G.Wagner,
M.Vasák,
J.H.Kägi,
K.Wüthrich.
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Ref.
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Eur J Biochem, 1987,
167,
457-466.
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PubMed id
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Secondary reference #3
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Title
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Metal co-Ordination in rat liver metallothionein-2 prepared with or without reconstitution of the metal clusters, And comparison with rabbit liver metallothionein-2.
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Authors
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M.Vasák,
E.Wörgötter,
G.Wagner,
J.H.Kägi,
K.Wüthrich.
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Ref.
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J Mol Biol, 1987,
196,
711-719.
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PubMed id
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