PDBsum entry 1m9o

Metal binding protein

PDB id: 1m9o

Contents

Protein chain

40 a.a. *

Metals

_ZN

* Residue conservation analysis

References listed in PDB file

Key reference

• Title: A cys3his zinc-Binding domain from nup475/tristetraprolin: a novel fold with a disklike structure.
• Authors: B.T.Amann, M.T.Worthington, J.M.Berg.
• Ref.: Biochemistry, 2003, 42, 217-221. [DOI no: 10.1021/bi026988m]
• PubMed id: 12515557

Abstract

Nup475 (also known as tristetraprolin and TIS11) includes two zinc-binding domains of the form Cys-X8-Cys-X5-Cys-X3-His. These domains are required for rapid degradation of tumor necrosis factor (TNF) and other mRNAs through the interaction with AU-rich elements in their 3'-untranslated regions. The three-dimensional solution structure of the first domain was determined by multidimensional nuclear magnetic resonance spectroscopy, revealing a novel fold around a central zinc ion. The core structure is disk-like with a diameter of approximately 25 A and a width of approximately 12 A. This structure provides a basis for evaluating the role of individual residues for structural stability and for nucleic acid binding.