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PDBsum entry 1m6r
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References listed in PDB file
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Key reference
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Title
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Crystal structure of rgd(cgcgcg): a z-Dna hexamer duplex with a 5'-(Rg) overhang.
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Authors
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B.Pan,
M.Sundaralingam.
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Ref.
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Acta Crystallogr D Biol Crystallogr, 2003,
59,
433-437.
[DOI no: ]
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PubMed id
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Abstract
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The crystal structure of the heptamer rGd(CGCGCG) has been determined at 1.54 A
resolution with R(work) and R(free) of 0.191 and 0.235, respectively. The
crystal belongs to space group P2(1)2(1)2(1), with unit-cell parameters a =
17.96, b = 31.47, c = 44.73 A and two independent strands in the asymmetric
unit. The chimera forms a Z-DNA hexamer duplex d(CGCGCG)(2), with the
5'-overhang rG invisible in the density. The replacement of rG in the 5'
terminus of d(GCGCGCG) changes the reverse Hoogsteen G.G base pairing of the
5'-overhang dG (Pan et al., 1997). However, the replacement does not change the
helix from Z-form to A-form or the water-structure motifs in the Z-DNA
double-helical structure.
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Figure 1.
Figure 1 (a) A-form duplex (gCGCGC)[2], (b) Z-form duplex
(CGCGCG)[2].
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Figure 4.
Figure 4 Hydration of the heptamer structure gCGCGCG. (a) Water
structure in the minor groove. The backbones of the heptamer are
shown in dark lines as zigzag chains, while hydrogen bonds are
shown as dotted lines. (b) Water structure in the major groove.
(c) Three different hydration patterns for the phosphate groups.
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The above figures are
reprinted
by permission from the IUCr:
Acta Crystallogr D Biol Crystallogr
(2003,
59,
433-437)
copyright 2003.
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Headers
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