PDBsum entry 1lyv

Hydrolase

PDB id: 1lyv

Contents

Protein chain

283 a.a. *

Ligands

PO4

Metals

_CL

Waters ×319

* Residue conservation analysis

PDB id:

1lyv

Name:

Hydrolase

Title:

High-resolution structure of the catalytically inactive yersinia tyrosine phosphatase c403a mutant in complex with phosphate.

Structure:

Protein-tyrosine phosphatase yoph. Chain: a. Fragment: ptpase domain. Synonym: virulence protein. Engineered: yes. Mutation: yes

Source:

Yersinia enterocolitica. Organism_taxid: 630. Gene: yoph. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Resolution:

1.36Å R-factor: 0.137 R-free: 0.190

Authors:

A.G.Evdokimov,D.S.Waugh,K.Routzahn,J.Tropea,S.Cherry

Key ref:

A.G.Evdokimov et al. High-Resolution structure of the catalytically inactive yersinia tyrosine phosphatase c403a mutant in complex with phosphate.. To be published, .

Date:

08-Jun-02 Release date: 03-Jul-02

Protein chain

P15273  (YOPH_YEREN) -  Tyrosine-protein phosphatase YopH from Yersinia enterocolitica

Seq: 468 a.a.

Struc: 283 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.3.1.3.48 - protein-tyrosine-phosphatase.
• Reaction: O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate

O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate (Bound ligand (Het Group name = PO4) corresponds exactly)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site