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PDBsum entry 1lyc
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Oxidoreductase
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PDB id
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1lyc
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Contents |
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* Residue conservation analysis
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References listed in PDB file
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Key reference
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Title
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Impact of the physical and chemical environment on the molecular structure of coprinus cinereus peroxidase.
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Authors
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K.Houborg,
P.Harris,
J.Petersen,
P.Rowland,
J.C.Poulsen,
P.Schneider,
J.Vind,
S.Larsen.
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Ref.
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Acta Crystallogr D Biol Crystallogr, 2003,
59,
989-996.
[DOI no: ]
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PubMed id
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Abstract
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The structure of the peroxidase from Coprinus cinereus (CiP) has been determined
in three different space groups and crystalline environments. Two of these are
of the recombinant glycosylated form (rCiP), which crystallized in space groups
P2(1)2(1)2(1) and C2. The third crystal form was obtained from a variant of CiP
in which the glycosylation sites have been removed (rCiPON). It crystallizes in
space group P2(1) with beta approximately 90 degrees; the structure was
determined from room-temperature data and low-temperature data obtained from
twinned crystals. Two independent molecules of CiP related by
non-crystallographic symmetry are contained in the three crystal forms. The
packing in the two structures of the glycosylated form of rCiP is closely
related, but differs from the packing in the unglycosylated rCiPON. A database
search based on small-molecule porphinato iron (III) complexes has been
performed and related to observations of the spin states and coordination
numbers of the iron ion. The room-temperature structures of CiP and one
structure of the almost identical peroxidase from Arthromyces ramosus (ARP) have
been used to identify 66 conserved water molecules and to assign a structural
role to most of them.
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Figure 5.
Figure 5 A structural representation of CiPON-RT with the
conserved water molecules in red (1028, 1027, 1009, 1023, 1034),
pink (1001, 1006, 1025, 1017), orange (1011, 1029, 1066, 1056)
and blue, the two calcium ions in green and the haem group in
black. The figure was prepared using the program MOLSCRIPT
(Kraulis, 1991[Kraulis, P. J. (1991). J. Appl. Cryst. 24,
946-950.]).
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Figure 6.
Figure 6 The water molecules found in the region around the haem
group in the CiP and ARP structures. The figure was prepared
using the program MOLSCRIPT (Kraulis, 1991[Kraulis, P. J.
(1991). J. Appl. Cryst. 24, 946-950.]).
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The above figures are
reprinted
by permission from the IUCr:
Acta Crystallogr D Biol Crystallogr
(2003,
59,
989-996)
copyright 2003.
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