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UniProt functional annotation for P55818 | ||
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| UniProt code: P55818. |
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| Organism: | |
Methylorubrum extorquens (strain ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1) (Methylobacterium extorquens). |
| Taxonomy: | |
Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales; Methylobacteriaceae; Methylorubrum. |
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| Function: | |
Catalyzes the dehydrogenation of methylene-H(4)MPT. Can also catalyze the reversible dehydrogenation of methylene-H(4)F with 20-fold lower catalytic efficiency. {ECO:0000269|PubMed:9765566}. |
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| Catalytic activity: | |
Reaction=5,10-methylenetetrahydromethanopterin + NADP(+) = 5,10- methenyl-5,6,7,8-tetrahydromethanopterin + NADPH; Xref=Rhea:RHEA:24682, ChEBI:CHEBI:57783, ChEBI:CHEBI:57818, ChEBI:CHEBI:58337, ChEBI:CHEBI:58349; Evidence={ECO:0000269|PubMed:9765566}; |
| Catalytic activity: | |
Reaction=(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + NADP(+) = 5,10- methenyltetrahydrofolate + NADPH; Xref=Rhea:RHEA:22812, ChEBI:CHEBI:15636, ChEBI:CHEBI:57455, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; EC=1.5.1.5; Evidence={ECO:0000269|PubMed:8144463, ECO:0000269|PubMed:9765566}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=20 mM for methylenetetrahydromethanopterin {ECO:0000269|PubMed:9765566}; KM=30 mM for methylenetetrahydrofolate {ECO:0000269|PubMed:9765566}; KM=30 mM for NADP(+) (in the presence of methylenetetrahydromethanopterin) {ECO:0000269|PubMed:9765566}; KM=10 mM for NADP(+) (in the presence of methylenetetrahydrofolate) {ECO:0000269|PubMed:9765566}; Vmax=600 umol/min/mg enzyme with methylenetetrahydromethanopterin as substrate {ECO:0000269|PubMed:9765566}; Vmax=30 umol/min/mg enzyme with methylenetetrahydrofolate as substrate {ECO:0000269|PubMed:9765566}; pH dependence: Optimum pH is 6.0. {ECO:0000269|PubMed:9765566}; Temperature dependence: Optimum temperature is 45 degrees Celsius. {ECO:0000269|PubMed:9765566}; |
| Pathway: | |
One-carbon metabolism; formaldehyde degradation; formate from formaldehyde (H(4)MPT route): step 2/5. {ECO:0000269|PubMed:9765566}. |
| Subunit: | |
Homotrimer. {ECO:0000269|PubMed:11297742}. |
| Subcellular location: | |
Cytoplasm {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.