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PDBsum entry 1lsl
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Cell adhesion PDB id
1lsl

 

 

 

 

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Contents
Protein chain
113 a.a. *
Ligands
FUL
FUC
Waters ×154
* Residue conservation analysis
PDB id:
1lsl
Name: Cell adhesion
Title: Crystal structure of the thrombospondin-1 type 1 repeats
Structure: Thrombospondin 1. Chain: a. Fragment: tsp type 1 repeats 2 and 3 (residues 434-546). Engineered: yes
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: thbs1. Expressed in: drosophila. Expression_system_taxid: 7215. Expression_system_cell: s2 cells.
Resolution:
1.90Å     R-factor:   0.238     R-free:   0.282
Authors: K.Tan,M.Duquette,J.Liu,Y.Dong,R.Zhang,A.Joachimiak,J.Lawler,J.-H.Wang
Key ref: K.Tan et al. (2002). Crystal structure of the TSP-1 type 1 repeats: a novel layered fold and its biological implication. J Cell Biol, 159, 373-382. PubMed id: 12391027
Date:
17-May-02     Release date:   18-Dec-02    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
P07996  (TSP1_HUMAN) -  Thrombospondin-1 from Homo sapiens
Seq:
Struc: 		 
Seq:
Struc: 		 
Seq:
Struc: 		1170 a.a.
113 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 

 
J Cell Biol 159:373-382 (2002)
PubMed id: 12391027  
 
 
Crystal structure of the TSP-1 type 1 repeats: a novel layered fold and its biological implication.
K.Tan, M.Duquette, J.H.Liu, Y.Dong, R.Zhang, A.Joachimiak, J.Lawler, J.H.Wang.
 
  ABSTRACT  
 
Thrombospondin-1 (TSP-1) contains three type 1 repeats (TSRs), which mediate cell attachment, glycosaminoglycan binding, inhibition of angiogenesis, activation of TGFbeta, and inhibition of matrix metalloproteinases. The crystal structure of the TSRs reported in this article reveals a novel, antiparallel, three-stranded fold that consists of alternating stacked layers of tryptophan and arginine residues from respective strands, capped by disulfide bonds on each end. The front face of the TSR contains a right-handed spiral, positively charged groove that might be the "recognition" face, mediating interactions with various ligands. This is the first high-resolution crystal structure of a TSR domain that provides a prototypic architecture for structural and functional exploration of the diverse members of the TSR superfamily.
 

Literature references that cite this PDB file's key reference

  PubMed id Reference
21262840 M.F.Bolliger, D.C.Martinelli, and T.C.Südhof (2011).
The cell-adhesion G protein-coupled receptor BAI3 is a high-affinity receptor for C1q-like proteins.
  Proc Natl Acad Sci U S A, 108, 2534-2539.  
21245295 S.Das, K.A.Owen, K.T.Ly, D.Park, S.G.Black, J.M.Wilson, C.D.Sifri, K.S.Ravichandran, P.B.Ernst, and J.E.Casanova (2011).
Brain angiogenesis inhibitor 1 (BAI1) is a pattern recognition receptor that mediates macrophage binding and engulfment of Gram-negative bacteria.
  Proc Natl Acad Sci U S A, 108, 2136-2141.  
20427418 A.A.Bentley, and J.C.Adams (2010).
The evolution of thrombospondins and their ligand-binding activities.
  Mol Biol Evol, 27, 2187-2197.  
20942952 A.Mongui, D.I.Angel, D.A.Moreno-Perez, S.Villarreal-Gonzalez, H.Almonacid, M.Vanegas, and M.A.Patarroyo (2010).
Identification and characterization of the Plasmodium vivax thrombospondin-related apical merozoite protein.
  Malar J, 9, 283.  
19947883 C.Kemper, J.P.Atkinson, and D.E.Hourcade (2010).
Properdin: emerging roles of a pattern-recognition molecule.
  Annu Rev Immunol, 28, 131-155.  
20425613 K.D.Hankenson, M.T.Sweetwyne, H.Shitaye, and K.L.Posey (2010).
Thrombospondins and novel TSR-containing proteins, R-spondins, regulate bone formation and remodeling.
  Curr Osteoporos Rep, 8, 68-76.  
19940141 K.Tsutsui, R.Manabe, T.Yamada, I.Nakano, Y.Oguri, D.R.Keene, G.Sengle, L.Y.Sakai, and K.Sekiguchi (2010).
ADAMTSL-6 is a novel extracellular matrix protein that binds to fibrillin-1 and promotes fibrillin-1 fibril formation.
  J Biol Chem, 285, 4870-4882.  
20684023 M.L.Tonkin, O.Grujic, M.Pearce, J.Crawford, and M.J.Boulanger (2010).
Structure of the micronemal protein 2 A/I domain from Toxoplasma gondii.
  Protein Sci, 19, 1985-1990.
PDB code: 2xgg
20593462 T.K.Tiefenbrunn, J.Blanco-Canosa, and P.E.Dawson (2010).
Alternative chemistries for the synthesis of thrombospondin-1 type 1 repeats.
  Biopolymers, 94, 405-413.  
18952191 K.B.Luther, and R.S.Haltiwanger (2009).
Role of unusual O-glycans in intercellular signaling.
  Int J Biochem Cell Biol, 41, 1011-1024.  
18409060 K.O.Yee, C.M.Connolly, M.Duquette, S.Kazerounian, R.Washington, and J.Lawler (2009).
The effect of thrombospondin-1 on breast cancer metastasis.
  Breast Cancer Res Treat, 114, 85-96.  
19424823 K.P.Holbourn, B.Perbal, and K.Ravi Acharya (2009).
Proteins on the catwalk: modelling the structural domains of the CCN family of proteins.
  J Cell Commun Signal, 3, 25-41.  
19671700 L.W.Wang, C.Leonhard-Melief, R.S.Haltiwanger, and S.S.Apte (2009).
Post-translational modification of thrombospondin type-1 repeats in ADAMTS-like 1/punctin-1 by C-mannosylation of tryptophan.
  J Biol Chem, 284, 30004-30015.  
19880749 M.Akiyama, S.Takeda, K.Kokame, J.Takagi, and T.Miyata (2009).
Crystal structures of the noncatalytic domains of ADAMTS13 reveal multiple discontinuous exosites for von Willebrand factor.
  Proc Natl Acad Sci U S A, 106, 19274-19279.
PDB codes: 3ghm 3ghn
19633296 M.L.Plassmeyer, K.Reiter, R.L.Shimp, S.Kotova, P.D.Smith, D.E.Hurt, B.House, X.Zou, Y.Zhang, M.Hickman, O.Uchime, R.Herrera, V.Nguyen, J.Glen, J.Lebowitz, A.J.Jin, L.H.Miller, N.J.MacDonald, Y.Wu, and D.L.Narum (2009).
Structure of the Plasmodium falciparum circumsporozoite protein, a leading malaria vaccine candidate.
  J Biol Chem, 284, 26951-26963.  
  19116307 R.Palla, S.Lavoretano, R.Lombardi, I.Garagiola, M.Karimi, A.Afrasiabi, M.Ramzi, R.De Cristofaro, and F.Peyvandi (2009).
The first deletion mutation in the TSP1-6 repeat domain of ADAMTS13 in a family with inherited thrombotic thrombocytopenic purpura.
  Haematologica, 94, 289-293.  
19385064 S.J.Li, T.Y.Yen, Y.Endo, M.Klauzinska, B.Baljinnyam, B.Macher, R.Callahan, and J.S.Rubin (2009).
Loss-of-function point mutations and two-furin domain derivatives provide insights about R-spondin2 structure and function.
  Cell Signal, 21, 916-925.  
19384999 T.K.Tiefenbrunn, and P.E.Dawson (2009).
Chemical synthesis and biotinylation of the thrombospondin domain TSR2.
  Protein Sci, 18, 970-979.  
19153605 Y.Li, C.Cao, W.Jia, L.Yu, M.Mo, Q.Wang, Y.Huang, J.M.Lim, M.Ishihara, L.Wells, P.Azadi, H.Robinson, Y.W.He, L.Zhang, and R.A.Mariuzza (2009).
Structure of the F-spondin domain of mindin, an integrin ligand and pattern recognition molecule.
  EMBO J, 28, 286-297.
PDB code: 3d34
19706610 Y.Liu, and D.F.Mosher (2009).
Interactions among stalk modules of thrombospondin-1.
  J Biol Chem, 284, 28563-28570.  
18070664 B.J.Mans, J.F.Andersen, I.M.Francischetti, J.G.Valenzuela, T.G.Schwan, V.M.Pham, M.K.Garfield, C.H.Hammer, and J.M.Ribeiro (2008).
Comparative sialomics between hard and soft ticks: implications for the evolution of blood-feeding behavior.
  Insect Biochem Mol Biol, 38, 42-58.  
18193164 C.B.Carlson, J.Lawler, and D.F.Mosher (2008).
Structures of thrombospondins.
  Cell Mol Life Sci, 65, 672-686.  
18692243 C.Kemper, and D.E.Hourcade (2008).
Properdin: New roles in pattern recognition and target clearance.
  Mol Immunol, 45, 4048-4056.  
18579773 C.Kemper, L.M.Mitchell, L.Zhang, and D.E.Hourcade (2008).
The complement protein properdin binds apoptotic T cells and promotes complement activation and phagocytosis.
  Proc Natl Acad Sci U S A, 105, 9023-9028.  
18789696 K.P.Holbourn, K.R.Acharya, and B.Perbal (2008).
The CCN family of proteins: structure-function relationships.
  Trends Biochem Sci, 33, 461-473.  
18602404 K.Tan, M.Duquette, J.H.Liu, J.Lawler, and J.H.Wang (2008).
The crystal structure of the heparin-binding reelin-N domain of f-spondin.
  J Mol Biol, 381, 1213-1223.
PDB code: 3coo
  19238248 L.Ayadi (2008).
Molecular modelling of the TSR domain of R-spondin 4.
  Bioinformation, 3, 119-123.  
17997122 M.E.Patarroyo, G.Cifuentes, and R.Rodríguez (2008).
Structural characterisation of sporozoite components for a multistage, multi-epitope, anti-malarial vaccine.
  Int J Biochem Cell Biol, 40, 543-557.  
  18512339 V.B.Carruthers, and F.M.Tomley (2008).
Microneme proteins in apicomplexans.
  Subcell Biochem, 47, 33-45.  
18614580 W.M.Woo, E.C.Berry, M.L.Hudson, R.E.Swale, A.Goncharov, and A.D.Chisholm (2008).
The C. elegans F-spondin family protein SPON-1 maintains cell adhesion in neural and non-neural tissues.
  Development, 135, 2747-2756.  
17407100 M.A.Rameix-Welti, C.H.Régnier, F.Bienaimé, J.Blouin, J.Schifferli, W.H.Fridman, C.Sautès-Fridman, and V.Frémeaux-Bacchi (2007).
Hereditary complement C7 deficiency in nine families: subtotal C7 deficiency revisited.
  Eur J Immunol, 37, 1377-1385.  
17126404 O.Meiniel, and A.Meiniel (2007).
The complex multidomain organization of SCO-spondin protein is highly conserved in mammals.
  Brain Res Rev, 53, 321-327.  
16900377 R.Didier, O.Meiniel, and A.Meiniel (2007).
Molecular cloning and early expression of chick embryo SCO-spondin.
  Cell Tissue Res, 327, 111-119.  
17216438 R.S.Schwarz, L.Hodes-Villamar, K.A.Fitzpatrick, M.G.Fain, A.L.Hughes, and L.F.Cadavid (2007).
A gene family of putative immune recognition molecules in the hydroid Hydractinia.
  Immunogenetics, 59, 233-246.  
17875744 S.Zisman, K.Marom, O.Avraham, L.Rinsky-Halivni, U.Gai, G.Kligun, V.Tzarfaty-Majar, T.Suzuki, and A.Klar (2007).
Proteolysis and membrane capture of F-spondin generates combinatorial guidance cues from a single molecule.
  J Cell Biol, 178, 1237-1249.  
17559888 X.Zhang, and J.Lawler (2007).
Thrombospondin-based antiangiogenic therapy.
  Microvasc Res, 74, 90-99.  
16406676 B.Ren, K.O.Yee, J.Lawler, and R.Khosravi-Far (2006).
Regulation of tumor angiogenesis by thrombospondin-1.
  Biochim Biophys Acta, 1765, 178-188.  
16420580 D.S.Annis, J.E.Murphy-Ullrich, and D.F.Mosher (2006).
Function-blocking antithrombospondin-1 monoclonal antibodies.
  J Thromb Haemost, 4, 459-468.  
16815922 H.Tossavainen, T.Pihlajamaa, T.K.Huttunen, E.Raulo, H.Rauvala, P.Permi, and I.Kilpeläinen (2006).
The layered fold of the TSR domain of P. falciparum TRAP contains a heparin binding site.
  Protein Sci, 15, 1760-1768.
PDB code: 2bbx
16736493 K.Pääkkönen, H.Tossavainen, P.Permi, H.Rakkolainen, H.Rauvala, E.Raulo, I.Kilpeläinen, and P.Güntert (2006).
Solution structures of the first and fourth TSR domains of F-spondin.
  Proteins, 64, 665-672.
PDB codes: 1szl 1vex
16407063 K.Tan, M.Duquette, J.H.Liu, R.Zhang, A.Joachimiak, J.H.Wang, and J.Lawler (2006).
The structures of the thrombospondin-1 N-terminal domain and its complex with a synthetic pentameric heparin.
  Structure, 14, 33-42.
PDB codes: 1z78 1za4 2erf
17082774 N.V.Lee, M.Sato, D.S.Annis, J.A.Loo, L.Wu, D.F.Mosher, and M.L.Iruela-Arispe (2006).
ADAMTS1 mediates the release of antiangiogenic polypeptides from TSP1 and 2.
  EMBO J, 25, 5270-5283.  
17146059 W.Gao, P.J.Anderson, E.M.Majerus, E.A.Tuley, and J.E.Sadler (2006).
Exosite interactions contribute to tension-induced cleavage of von Willebrand factor by the antithrombotic ADAMTS13 metalloprotease.
  Proc Natl Acad Sci U S A, 103, 19099-19104.  
16084754 F.Brossier, and L.David Sibley (2005).
Toxoplasma gondii: microneme protein MIC2.
  Int J Biochem Cell Biol, 37, 2266-2272.  
16147889 G.M.Ye, C.M.Chen, C.Chen, W.B.Yu, X.Gu, C.Q.Wu, J.H.Guo, and S.Y.Zhao (2005).
Human THROMBOSPONDIN-1 gene contains a natural antisense transcript, and characterization of its expression in human multiple tissues and cells.
  DNA Seq, 16, 295-299.  
16869777 M.A.Grant, and R.Kalluri (2005).
Structural basis for the functions of endogenous angiogenesis inhibitors.
  Cold Spring Harb Symp Quant Biol, 70, 399-410.  
15716381 S.M.Short, A.Derrien, R.P.Narsimhan, J.Lawler, D.E.Ingber, and B.R.Zetter (2005).
Inhibition of endothelial cell migration by thrombospondin-1 type-1 repeats is mediated by beta1 integrins.
  J Cell Biol, 168, 643-653.  
15094109 J.C.Adams, and J.Lawler (2004).
The thrombospondins.
  Int J Biochem Cell Biol, 36, 961-968.  
15094119 J.Lawler, and M.Detmar (2004).
Tumor progression: the effects of thrombospondin-1 and -2.
  Int J Biochem Cell Biol, 36, 1038-1045.  
  15277228 K.O.Yee, M.Streit, T.Hawighorst, M.Detmar, and J.Lawler (2004).
Expression of the type-1 repeats of thrombospondin-1 inhibits tumor growth through activation of transforming growth factor-beta.
  Am J Pathol, 165, 541-552.  
15014436 M.Kvansakul, J.C.Adams, and E.Hohenester (2004).
Structure of a thrombospondin C-terminal fragment reveals a novel calcium core in the type 3 repeats.
  EMBO J, 23, 1223-1233.
PDB code: 1ux6
15094110 R.P.Tucker (2004).
The thrombospondin type 1 repeat superfamily.
  Int J Biochem Cell Biol, 36, 969-974.  
15473834 S.H.Kappe, C.A.Buscaglia, and V.Nussenzweig (2004).
Plasmodium sporozoite molecular cell biology.
  Annu Rev Cell Dev Biol, 20, 29-59.  
15094111 Y.Feinstein, and A.Klar (2004).
The neuronal class 2 TSR proteins F-spondin and Mindin: a small family with divergent biological activities.
  Int J Biochem Cell Biol, 36, 975-980.  
14561772 N.Kamei, and C.G.Glabe (2003).
The species-specific egg receptor for sea urchin sperm adhesion is EBR1,a novel ADAMTS protein.
  Genes Dev, 17, 2502-2507.  
12403807 R.L.Silverstein (2002).
The face of TSR revealed: an extracellular signaling domain is exposed.
  J Cell Biol, 159, 203-206.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.

 

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