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PDBsum entry 1lm7
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protein Protein-protein interface(s) links
Structural protein PDB id
1lm7

 

 

 

 

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Contents
Protein chain
238 a.a. *
* Residue conservation analysis
PDB id:
1lm7
Name: Structural protein
Title: Structures of two intermediate filament-binding fragments of desmoplakin reveal a unique repeat motif structure
Structure: Subdomain of desmoplakin carboxy-terminal domain (dpct). Chain: a, b. Fragment: residues 2209-2456. Engineered: yes
Source: Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 562.
Biol. unit: Dimer (from PQS)
Resolution:
3.00Å     R-factor:   0.249     R-free:   0.296
Authors: H.J.Choi,S.Park-Snyder,L.T.Pascoe,K.J.Green,W.I.Weis
Key ref:
H.J.Choi et al. (2002). Structures of two intermediate filament-binding fragments of desmoplakin reveal a unique repeat motif structure. Nat Struct Biol, 9, 612-620. PubMed id: 12101406
Date:
30-Apr-02     Release date:   31-Jul-02    
PROCHECK
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 Headers
 References

Protein chains
Pfam   ArchSchema ?
P15924  (DESP_HUMAN) -  Desmoplakin from Homo sapiens
Seq:
Struc: 		 
Seq:
Struc: 		 
Seq:
Struc: 		 
Seq:
Struc: 		 
Seq:
Struc: 		 
Seq:
Struc: 		2871 a.a.
238 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 

 
Nat Struct Biol 9:612-620 (2002)
PubMed id: 12101406  
 
 
Structures of two intermediate filament-binding fragments of desmoplakin reveal a unique repeat motif structure.
H.J.Choi, S.Park-Snyder, L.T.Pascoe, K.J.Green, W.I.Weis.
 
  ABSTRACT  
 
Desmosomes are intercellular junctions in which cadherin cell adhesion molecules are linked to the intermediate filament (IF) system. Desmoplakin is a member of the plakin family of IF-binding proteins. The C-terminal domain of desmoplakin (DPCT) mediates binding to IFs in desmosomes. The DPCT sequence contains three regions, termed A, B and C, consisting of 4.5 copies of a 38-amino acid repeat motif. We demonstrate that these regions form discrete subdomains that bind to IFs and report the crystal structures of domains B and C. In contrast to the elongated structures formed by other kinds of repeat motifs, the plakin repeats form a globular structure with a unique fold. A conserved basic groove found on the domain may represent an IF-binding site.
 

Literature references that cite this PDB file's key reference

  PubMed id Reference
20626351 H.A.Thomason, A.Scothern, S.McHarg, and D.R.Garrod (2010).
Desmosomes: adhesive strength and signalling in health and disease.
  Biochem J, 429, 419-433.  
19768769 J.G.Boyer, M.A.Bernstein, and C.Boudreau-Larivière (2010).
Plakins in striated muscle.
  Muscle Nerve, 41, 299-308.  
20554761 L.M.Godsel, A.D.Dubash, A.E.Bass-Zubek, E.V.Amargo, J.L.Klessner, R.P.Hobbs, X.Chen, and K.J.Green (2010).
Plakophilin 2 couples actomyosin remodeling to desmosomal plaque assembly via RhoA.
  Mol Biol Cell, 21, 2844-2859.  
19924139 M.G.Mahoney, S.Sadowski, D.Brennan, P.Pikander, P.Saukko, J.Wahl, H.Aho, K.Heikinheimo, L.Bruckner-Tuderman, A.Fertala, J.Peltonen, J.Uitto, and S.Peltonen (2010).
Compound heterozygous desmoplakin mutations result in a phenotype with a combination of myocardial, skin, hair, and enamel abnormalities.
  J Invest Dermatol, 130, 968-978.  
20613772 R.P.Hobbs, S.Y.Han, P.A.van der Zwaag, M.C.Bolling, J.D.Jongbloed, M.F.Jonkman, S.Getsios, A.S.Paller, and K.J.Green (2010).
Insights from a desmoplakin mutation identified in lethal acantholytic epidermolysis bullosa.
  J Invest Dermatol, 130, 2680-2683.  
19955337 B.V.Desai, R.M.Harmon, and K.J.Green (2009).
Desmosomes at a glance.
  J Cell Sci, 122, 4401-4407.  
  19736524 J.M.de Pereda, E.Ortega, N.Alonso-García, M.Gómez-Hernández, and A.Sonnenberg (2009).
Advances and perspectives of the architecture of hemidesmosomes: lessons from structural biology.
  Cell Adh Migr, 3, 361-364.  
18386043 J.Waschke (2008).
The desmosome and pemphigus.
  Histochem Cell Biol, 130, 21-54.  
17945476 D.L.Stokes (2007).
Desmosomes from a structural perspective.
  Curr Opin Cell Biol, 19, 565-571.  
17161423 J.J.Jefferson, C.Ciatto, L.Shapiro, and R.K.Liem (2007).
Structural analysis of the plakin domain of bullous pemphigoid antigen1 (BPAG1) suggests that plakins are members of the spectrin superfamily.
  J Mol Biol, 366, 244-257.
PDB code: 2iak
17224453 R.Spurny, K.Abdoulrahman, L.Janda, D.Rünzler, G.Köhler, M.J.Castañón, and G.Wiche (2007).
Oxidation and nitrosylation of cysteines proximal to the intermediate filament (IF)-binding site of plectin: effects on structure and vimentin binding and involvement in IF collapse.
  J Biol Chem, 282, 8175-8187.  
15654952 A.E.Kalinin, A.E.Kalinin, M.Aho, J.Uitto, and S.Aho (2005).
Breaking the connection: caspase 6 disconnects intermediate filament-binding domain of periplakin from its actin-binding N-terminal region.
  J Invest Dermatol, 124, 46-55.  
16365169 L.M.Godsel, S.N.Hsieh, E.V.Amargo, A.E.Bass, L.T.Pascoe-McGillicuddy, A.C.Huen, M.E.Thorne, C.A.Gaudry, J.K.Park, K.Myung, R.D.Goldman, T.L.Chew, and K.J.Green (2005).
Desmoplakin assembly dynamics in four dimensions: multiple phases differentially regulated by intermediate filaments and actin.
  J Cell Biol, 171, 1045-1059.  
16175511 M.F.Jonkman, A.M.Pasmooij, S.G.Pasmans, M.P.van den Berg, H.J.Ter Horst, A.Timmer, and H.H.Pas (2005).
Loss of desmoplakin tail causes lethal acantholytic epidermolysis bullosa.
  Am J Hum Genet, 77, 653-660.  
15189158 H.Herrmann, and U.Aebi (2004).
Intermediate filaments: molecular structure, assembly mechanism, and integration into functionally distinct intracellular Scaffolds.
  Annu Rev Biochem, 73, 749-789.  
15561586 T.Yin, and K.J.Green (2004).
Regulation of desmosome assembly and adhesion.
  Semin Cell Dev Biol, 15, 665-677.  
12791695 D.Spazierer, P.Fuchs, V.Pröll, L.Janda, S.Oehler, I.Fischer, R.Hauptmann, and G.Wiche (2003).
Epiplakin gene analysis in mouse reveals a single exon encoding a 725-kDa protein with expression restricted to epithelial tissues.
  J Biol Chem, 278, 31657-31666.  
14517208 K.Röper, and N.H.Brown (2003).
Maintaining epithelial integrity: a function for gigantic spectraplakin isoforms in adherens junctions.
  J Cell Biol, 162, 1305-1315.  
12802069 L.Fontao, B.Favre, S.Riou, D.Geerts, F.Jaunin, J.H.Saurat, K.J.Green, A.Sonnenberg, and L.Borradori (2003).
Interaction of the bullous pemphigoid antigen 1 (BP230) and desmoplakin with intermediate filaments is mediated by distinct sequences within their COOH terminus.
  Mol Biol Cell, 14, 1978-1992.  
12145641 P.A.Coulombe (2002).
A new fold on an old story: attachment of intermediate filaments to desmosomes.
  Nat Struct Biol, 9, 560-562.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.

 

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