PDBsum entry 1lk9

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Lyase PDB id
Protein chains
425 a.a. *
SO4 ×11
EPE ×2
_CL ×2
Waters ×830
* Residue conservation analysis

References listed in PDB file
Key reference
Title The active principle of garlic at atomic resolution.
Authors E.B.Kuettner, R.Hilgenfeld, M.S.Weiss.
Ref. J Biol Chem, 2002, 277, 46402-46407. [DOI no: 10.1074/jbc.M208669200]
PubMed id 12235163
Despite the fact that many cultures around the world value and utilize garlic as a fundamental component of their cuisine as well as of their medicine cabinets, relatively little is known about the plant's protein configuration that is responsible for the specific properties of garlic. Here, we report the three-dimensional structure of the garlic enzyme alliinase at 1.5 A resolution. Alliinase constitutes the major protein component in garlic bulbs, and it is able to cleave carbon-sulfur bonds. The active enzyme is a pyridoxal-5'-phosphate-dependent homodimeric glycoprotein and belongs to the class I family of pyridoxal-5'-phosphate-dependent enzymes. In addition, it contains a novel epidermal growth factor-like domain that makes it unique among all pyridoxal-5'-phosphate-dependent enzymes.
Figure 1.
Fig. 1. A, therapeutically active sulfur compounds from garlic; a representative for each of the three substance classes (allyl sulfides, dithiines, and ajoenes) is shown. B, the enzymatic reaction catalyzed by alliinase.
Figure 6.
Fig. 6. The active site of alliinase from garlic. A, stereo diagram of all residues in hydrogen bonding distance to either the cofactor PLP ( yellow) or the modeled substrate alliin. B, schematic diagram of the active site architecture. Both alliin configurations are shown: (+)-alliin, (magenta) and ( )-alliin, (cyan). Hydrogen bonding distances are given in Å.
The above figures are reprinted by permission from the ASBMB: J Biol Chem (2002, 277, 46402-46407) copyright 2002.
Secondary reference #1
Title Purification, Characterization, And crystallization of alliinase from garlic.
Authors E.B.Kuettner, R.Hilgenfeld, M.S.Weiss.
Ref. Arch Biochem Biophys, 2002, 402, 192-200. [DOI no: 10.1016/S0003-9861(02)00088-7]
PubMed id 12051663
Full text Abstract
Secondary reference #2
Title Erratum to ``purification, Characterization and crystallization of alliinase from garlic. [Arch. Biochem. Biophys. 402, 192-200.]''
Authors E.B.Kuettner, R.Hilgenfeld, M.S.Weiss.
Ref. arch biochem biophys, 2002, 404, 339.
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