PDBsum entry 1lfn

Transferase

PDB id: 1lfn

Contents

Protein chain

305 a.a.

Theoretical model

PDB id:

1lfn

Name:

Transferase

Title:

Theoretical structure of human-cyclin dependent kinase 3 (cdk3)

Structure:

Cell division protein kinase 3. Chain: a. Synonym: cyclin-dependent kinase 3, cdk3. Ec: 2.7.1.-

Source:

Homo sapiens. Human

Authors:

K.Narayana,T.Rajasekhar,J.A.R.P.Sarma

Key ref:

G.E.Norris et al. (1991). Molecular replacement solution of the structure of apolactoferrin, a protein displaying large-scale conformational change. Acta Crystallogr B, 47, 998. PubMed id: 1772635

Date:

11-Apr-02 Release date: 24-Apr-02

Protein chain

Q00526  (CDK3_HUMAN) -  Cyclin-dependent kinase 3

Seq: 305 a.a.

Struc: 305 a.a.

Acta Crystallogr B 47:998 (1991)

PubMed id: 1772635

Molecular replacement solution of the structure of apolactoferrin, a protein displaying large-scale conformational change.

G.E.Norris, B.F.Anderson, E.N.Baker.

ABSTRACT

The crystal structure of an orthorhombic form of human apolactoferrin (ApoLf) has been determined from 2.8 A diffractometer data by molecular replacement methods. A variety of search models derived from the diferric lactoferrin structure (Fe2Lf) were used to obtain a consistent solution to the rotation function. An R-factor search gave the correct translational solution and the model was refined by rigid-body least-squares refinement (program CORELS). Only three of the four domains were located correctly by this procedure, however; the fourth was finally placed correctly by rotating it manually onto three strands of electron density which were recognized as part of its central beta-sheet. The final model, refined by restrained least-squares methods to an R factor of 0.214 for data in the resolution range 10.0 to 2.8 A, shows a large domain movement in the N-terminal half of the molecule (a 54 degree rotation of domain N2) and smaller domain movements elsewhere, when compared with Fe2Lf. A feature of the crystal structure is that although the ApoLf and Fe2Lf unit cells appear very similar, their crystal packing and molecular structures are quite different.