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UniProt functional annotation for P00344 | ||
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| UniProt code: P00344. |
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| Organism: | |
Geobacillus stearothermophilus (Bacillus stearothermophilus). |
| Taxonomy: | |
Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Geobacillus. |
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| Function: | |
Catalyzes the conversion of lactate to pyruvate. {ECO:0000255|HAMAP-Rule:MF_00488, ECO:0000269|PubMed:3580377, ECO:0000269|Ref.5}. |
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| Catalytic activity: | |
Reaction=(S)-lactate + NAD(+) = H(+) + NADH + pyruvate; Xref=Rhea:RHEA:23444, ChEBI:CHEBI:15361, ChEBI:CHEBI:15378, ChEBI:CHEBI:16651, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.1.1.27; Evidence={ECO:0000255|HAMAP-Rule:MF_00488, ECO:0000305|PubMed:3580377}; |
| Activity regulation: | |
Allosterically activated by fructose 1,6- bisphosphate (FBP). The improvement in affinity for substrate occurs in two steps; the binding of fructose 1,6-bisphosphate (FBP) to the dimer, and the dimer to tetramer conversion. {ECO:0000269|PubMed:3580377, ECO:0000269|Ref.5, ECO:0000305|PubMed:1731077}. |
| Pathway: | |
Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1. {ECO:0000255|HAMAP-Rule:MF_00488}. |
| Subunit: | |
Exists as a dimer and a tetramer (dimer of dimers). The conversion occurs via the binding of fructose 1,6-bisphosphate (FBP) to the dimer. {ECO:0000269|PubMed:1731077, ECO:0000269|PubMed:2330370, ECO:0000269|PubMed:3580377, ECO:0000305|Ref.5}. |
| Subcellular location: | |
Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00488}. |
| Similarity: | |
Belongs to the LDH/MDH superfamily. LDH family. {ECO:0000255|HAMAP-Rule:MF_00488, ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.