PDBsum entry 1l0q

Protein binding

PDB id: 1l0q

Contents

Protein chains

391 a.a. *

Waters ×1364

* Residue conservation analysis

PDB id:

1l0q

Name:

Protein binding

Title:

Tandem yvtn beta-propeller and pkd domains from an archaeal surface layer protein

Structure:

Surface layer protein. Chain: a, b, c, d. Fragment: yvtn beta-propeller domain 1 and pkd domain 1. Engineered: yes

Source:

Methanosarcina mazei. Organism_taxid: 213585. Strain: s-6. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

2.40Å R-factor: 0.214 R-free: 0.263

Authors:

H.Jing,J.Takagi,J.-H.Liu,S.Lindgren,R.-G.Zhang,A.Joachimiak,J.- H.Wang,T.A.Springer

Key ref:

H.Jing et al. (2002). Archaeal surface layer proteins contain beta propeller, PKD, and beta helix domains and are related to metazoan cell surface proteins. Structure, 10, 1453-1464. PubMed id: 12377130 DOI: 10.1016/S0969-2126(02)00840-7

Date:

12-Feb-02 Release date: 06-Nov-02

Protein chains

Q50245  (Q50245_METMZ) -  ORF492 from Methanosarcina mazei

Seq: 491 a.a.

Struc: 391 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

DOI no: 10.1016/S0969-2126(02)00840-7

Structure 10:1453-1464 (2002)

PubMed id: 12377130

Archaeal surface layer proteins contain beta propeller, PKD, and beta helix domains and are related to metazoan cell surface proteins.

H.Jing, J.Takagi, J.H.Liu, S.Lindgren, R.G.Zhang, A.Joachimiak, J.H.Wang, T.A.Springer.

ABSTRACT

The surface layer of archaeobacteria protects cells from extreme environments and, in Methanosarcina, may regulate cell adhesion. We identify three domain types that account for the complete architecture of numerous Methanosarcina surface layer proteins (SLPs). We solve the crystal structure for two of these domains, which correspond to the two N-terminal domains of an M. mazei SLP. One domain displays a unique, highly symmetrical, seven-bladed beta propeller fold, and the other belongs to the polycystic kidney disease (PKD) superfamily fold. The third domain is predicted to adopt a beta helix fold. These domains have homologs in metazoan cell surface proteins, suggesting remarkable relationships between domains in archaeal SLPs and metazoan cell surface proteins.

Selected figure(s)

Figure 1.
Figure 1. Overall Structure(A) Schematic domain structure of the full-length M. mazei SLP.(B) Ribbons diagram of YVTN b propeller (magenta) and PKD (blue) domains as viewed from the top of the b propeller domain. Domain linkers, white; bulge on strand 1 of W7, green.(C) Side view of the Ca traces of four molecules in the asymmetric unit, superimposed on the b propeller domain.(D) Charges on the bumpy bottom surface of the b propeller domain. The GRASP representation of surface charge is colored from red ( -10 kEV) to blue (+10 kEV). The view is vert, similar 90 degrees from (C) and vert, similar 180 degrees from (B).

The above figure is reprinted by permission from Cell Press: Structure (2002, 10, 1453-1464) copyright 2002.

Figure was selected by an automated process.