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UniProt functional annotation for P08311 | ||
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| UniProt code: P08311. |
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| Organism: | |
Homo sapiens (Human). |
| Taxonomy: | |
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. |
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| Function: | |
Serine protease with trypsin- and chymotrypsin-like specificity. Cleaves complement C3. Has antibacterial activity against the Gram-negative bacterium P.aeruginosa, antibacterial activity is inhibited by LPS from P.aeruginosa, Z-Gly-Leu-Phe-CH2Cl and phenylmethylsulfonyl fluoride. {ECO:0000269|PubMed:1861080, ECO:0000269|PubMed:1937776, ECO:0000269|PubMed:8194606}. |
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| Catalytic activity: | |
Reaction=Specificity similar to chymotrypsin C.; EC=3.4.21.20; |
| Activity regulation: | |
Inhibited by soybean trypsin inhibitor, benzamidine, the synthetic peptide R13K, Z-Gly-Leu-Phe-CH2Cl, phenylmethylsulfonyl fluoride, 3,4-dichloroisocoumarin, DFP, SBTI and alpha-1-antitrypsin. Inhibited by LPS from P.aeruginosa but not by LPS from S.minnesota. Not inhibited by elastinal, CMK, TLCK and ETDA. {ECO:0000269|PubMed:1861080, ECO:0000269|PubMed:1937776, ECO:0000269|PubMed:8194606}. |
| Biophysicochemical properties: | |
Kinetic parameters: KM=1.15 mM for Z-Lys-SBzl {ECO:0000269|PubMed:8194606}; KM=0.26 mM for Suc-Ala-Ala-Pro-Phe-SBzl {ECO:0000269|PubMed:8194606}; |
| Subunit: | |
(Microbial infection) Interacts with M.tuberculosis protein Rv3364c. {ECO:0000269|PubMed:22275911}. |
| Subcellular location: | |
Cell surface {ECO:0000269|PubMed:8194606}. |
| Similarity: | |
Belongs to the peptidase S1 family. {ECO:0000255|PROSITE- ProRule:PRU00274}. |
Annotations taken from UniProtKB at the EBI.