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PDBsum entry 1ktq
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Nucleotidyltransferase
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PDB id
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1ktq
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References listed in PDB file
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Key reference
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Title
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Crystal structure of the large fragment of thermus aquaticus DNA polymerase i at 2.5-A resolution: structural basis for thermostability.
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Authors
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S.Korolev,
M.Nayal,
W.M.Barnes,
E.Di cera,
G.Waksman.
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Ref.
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Proc Natl Acad Sci U S A, 1995,
92,
9264-9268.
[DOI no: ]
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PubMed id
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Abstract
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The crystal structure of the large fragment of the Thermus aquaticus DNA
polymerase (Klentaq1), determined at 2.5-A resolution, demonstrates a compact
two-domain architecture. The C-terminal domain is identical in fold to the
equivalent region of the Klenow fragment of Escherichia coli DNA polymerase I
(Klenow pol I). Although the N-terminal domain of Klentaq1 differs greatly in
sequence from its counterpart in Klenow pol I, it has clearly evolved from a
common ancestor. The structure of Klentaq1 reveals the strategy utilized by this
protein to maintain activity at high temperatures and provides the structural
basis for future improvements of the enzyme.
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