spacer
spacer

PDBsum entry 1kq3

Go to PDB code: 
Top Page protein ligands metals links
Oxidoreductase PDB id
1kq3
Jmol
Contents
Protein chain
364 a.a. *
Ligands
TRS
Metals
_ZN
_CL
Waters ×227
* Residue conservation analysis

References listed in PDB file
Key reference
Title Structural genomics of the thermotoga maritima proteome implemented in a high-Throughput structure determination pipeline.
Authors S.A.Lesley, P.Kuhn, A.Godzik, A.M.Deacon, I.Mathews, A.Kreusch, G.Spraggon, H.E.Klock, D.Mcmullan, T.Shin, J.Vincent, A.Robb, L.S.Brinen, M.D.Miller, T.M.Mcphillips, M.A.Miller, D.Scheibe, J.M.Canaves, C.Guda, L.Jaroszewski, T.L.Selby, M.A.Elsliger, J.Wooley, S.S.Taylor, K.O.Hodgson, I.A.Wilson, P.G.Schultz, R.C.Stevens.
Ref. Proc Natl Acad Sci U S A, 2002, 99, 11664-11669. [DOI no: 10.1073/pnas.142413399]
PubMed id 12193646
Abstract
Structural genomics is emerging as a principal approach to define protein structure-function relationships. To apply this approach on a genomic scale, novel methods and technologies must be developed to determine large numbers of structures. We describe the design and implementation of a high-throughput structural genomics pipeline and its application to the proteome of the thermophilic bacterium Thermotoga maritima. By using this pipeline, we successfully cloned and attempted expression of 1,376 of the predicted 1,877 genes (73%) and have identified crystallization conditions for 432 proteins, comprising 23% of the T. maritima proteome. Representative structures from TM0423 glycerol dehydrogenase and TM0449 thymidylate synthase-complementing protein are presented as examples of final outputs from the pipeline.
Figure 1.
Fig. 1. T. maritima structural genomics pipeline.
Figure 2.
Fig. 2. Custom instrumentation used to process T. maritima proteome. (A) A 96-tube fermentor for high-cell density growth. (B) Purification robot that processes cell pellets from fermentation through affinity purification. (C) Nano-drop crystallization robotics used to set up crystal trials. (D) Plate imaging robotics used to analyze crystal trials.
PROCHECK
Go to PROCHECK summary
 Headers