 |
PDBsum entry 1kef
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Protein binding
|
PDB id
|
|
|
|
1kef
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
Contents |
 |
|
|
|
|
|
|
|
|
* Residue conservation analysis
|
|
|
|
|
References listed in PDB file
|
|
|
Key reference
|
|
|
Title
|
|
The pdz1 domain of sap90. Characterization of structure and binding.
|
|
|
Authors
|
|
A.Piserchio,
M.Pellegrini,
S.Mehta,
S.M.Blackman,
E.P.Garcia,
J.Marshall,
D.F.Mierke.
|
|
|
Ref.
|
|
J Biol Chem, 2002,
277,
6967-6973.
[DOI no: ]
|
|
|
PubMed id
|
|
|
|
|
|
Abstract
|
|
|
The structural features of the PDZ1 domain of the synapse-associated protein
SAP90 have been characterized by NMR. A comparison with the structures of the
PDZ2 and PDZ3 domains of SAP90 illustrates significant differences, which may
account for the unique binding properties of these homologous domains. Within
the postsynaptic density, SAP90 functions as a molecular scaffold with a number
of the protein-protein interactions mediated through the PDZ1 domain. Here,
using fluorescence anisotropy and NMR chemical shift analysis, we have
characterized the association of PDZ1 to the C-terminal peptides of the GluR6
subunit of the kainate receptor, voltage-gated K(+) channel Kv1.4, and
microtubule-associate protein CRIPT, all of which are known to associate with
SAP90. The latter two, which possess the consensus sequence for binding to PDZ
domains (T/S-X-V-oh), have low micromolar binding affinities (1.5-15 microm).
The C terminus of GluR6, RLPGKETMA-oh, lacking the consensus sequence, binds to
PDZ1 of SAP90 with an affinity of 160 microm. The NMR data illustrate that
although all three peptides occupy the binding groove capped by the GLGF loop of
PDZ1, specific differences are present, consistent with the variation in binding
affinities.
|
|
|
|
|
|
|
|
Figure 1.
Fig. 1. 1H-15N HSQC spectrum of the PDZ1 domain of SAP90.
Collected at 35 °C, pH 6.8 at 600 MHz.
|
|
Figure 3.
Fig. 3. One representative structure of the PDZ1 domain
of SAP90 with the secondary structure elements highlighted.
|
|
|
|
|
|
The above figures are
reprinted
by permission from the ASBMB:
J Biol Chem
(2002,
277,
6967-6973)
copyright 2002.
|
|
|
|
|
|
|
