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UniProt functional annotation for Q8U3L0 | ||
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| UniProt code: Q8U3L0. |
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| Organism: | |
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1). |
| Taxonomy: | |
Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae; Pyrococcus. |
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| Function: | |
Broad specificity carboxypetidase that releases amino acids sequentially from the C-terminus, including neutral, aromatic, polar and basic residues, but not Pro, Gly, Asp and Glu. {ECO:0000269|PubMed:10595552}. |
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| Catalytic activity: | |
Reaction=Release of a C-terminal amino acid with broad specificity, except for -Pro.; EC=3.4.17.19; Evidence={ECO:0000269|PubMed:10595552}; |
| Cofactor: | |
Name=Co(2+); Xref=ChEBI:CHEBI:48828; Evidence={ECO:0000269|PubMed:10595552, ECO:0000269|PubMed:11839307}; Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000269|PubMed:10595552, ECO:0000269|PubMed:11839307}; Note=Binds 1 cobalt ion per subunit. Can also utilize Mn(2+) (in vitro). Is not active with zinc ions. {ECO:0000269|PubMed:10595552, ECO:0000269|PubMed:11839307}; |
| Activity regulation: | |
EDTA and DTT reversibly abolish carboxypeptidase activity. {ECO:0000269|PubMed:10595552}. |
| Biophysicochemical properties: | |
pH dependence: Optimum pH is 6.2-6.6. {ECO:0000269|PubMed:10595552}; Temperature dependence: Optimum temperature is 90-100 degrees Celsius. {ECO:0000269|PubMed:10595552}; |
| Subunit: | |
Homodimer. {ECO:0000269|PubMed:10595552, ECO:0000269|PubMed:11839307}. |
| Similarity: | |
Belongs to the peptidase M32 family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.