 |
PDBsum entry 1k66
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Signaling protein
|
PDB id
|
|
|
|
1k66
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
Contents |
 |
|
|
|
|
|
|
|
|
|
|
|
|
* Residue conservation analysis
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
|
|
|
|
|
|
| |
|
|
Biophys J
87:476-487
(2004)
|
|
PubMed id:
|
|
|
|
|
|
| |
|
Crystal structures of two cyanobacterial response regulators in apo- and phosphorylated form reveal a novel dimerization motif of phytochrome-associated response regulators.
|
|
C.Benda,
C.Scheufler,
N.Tandeau de Marsac,
W.Gärtner.
|
|
|
|
|
| |
ABSTRACT
|
|
|
|
| |
|
|
The structures of two response regulators (RRs) from the cyanobacterium
Calothrix PCC7601, RcpA and RcpB, were solved to 1.9- and 1.75-A resolution,
respectively. RcpA was found in phosphorylated and RcpB in nonphosphorylated
form. Both RRs are members of phytochrome-associated, light-sensing
two-component signal transduction pathways, based on histidine kinase-mediated
receptor autophosphorylation and phosphorelay to a RR. Despite the overall
folding similarity to CheY-type RRs ((beta/alpha)(5)-motif), RcpA and RcpB form
homodimers, irrespective of their phosphorylation state, giving insight into a
signal transduction putatively different from that of other known RRs.
Dimerization is accomplished by a C-terminal extension of the RR polypeptide
chain, and the surface formed by H4, beta 5, and H5, which constitute a
hydrophobic contact area with distinct interactions between residues of either
subunit. Sequence alignments reveal that the identified dimerization motif is
archetypal for phytochrome-associated RRs, making them a novel subgroup of
CheY-type RRs. The protein structures of RcpA and RcpB are compared to the
recently presented protein structure of Rcp1 from Synechocystis.
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
 |
 |
|
Literature references that cite this PDB file's key reference
|
|
|
| |
PubMed id
|
|
Reference
|
|
|
|
|
|
R.Gao,
and
A.M.Stock
(2010).
Molecular strategies for phosphorylation-mediated regulation of response regulator activity.
|
| |
Curr Opin Microbiol,
13,
160-167.
|
|
|
|
|
|
|
S.Sharda,
M.S.Koay,
Y.J.Kim,
M.Engelhard,
and
W.Gärtner
(2009).
A non-hydrolyzable ATP derivative generates a stable complex in a light-inducible two-component system.
|
| |
J Biol Chem,
284,
33999-34004.
|
|
|
|
|
|
|
B.Quest,
T.Hübschmann,
S.Sharda,
N.Tandeau de Marsac,
and
W.Gärtner
(2007).
Homologous expression of a bacterial phytochrome. The cyanobacterium Fremyella diplosiphon incorporates biliverdin as a genuine, functional chromophore.
|
| |
FEBS J,
274,
2088-2098.
|
|
|
|
|
|
|
J.Purschwitz,
S.Müller,
C.Kastner,
and
R.Fischer
(2006).
Seeing the rainbow: light sensing in fungi.
|
| |
Curr Opin Microbiol,
9,
566-571.
|
|
|
|
|
|
|
K.I.Varughese,
I.Tsigelny,
and
H.Zhao
(2006).
The crystal structure of beryllofluoride Spo0F in complex with the phosphotransferase Spo0B represents a phosphotransfer pretransition state.
|
| |
J Bacteriol,
188,
4970-4977.
|
|
|
PDB code:
|
|
|
|
|
|
|
|
R.Gao,
A.Mukhopadhyay,
F.Fang,
and
D.G.Lynn
(2006).
Constitutive activation of two-component response regulators: characterization of VirG activation in Agrobacterium tumefaciens.
|
| |
J Bacteriol,
188,
5204-5211.
|
|
|
|
|
|
|
K.I.Varughese
(2005).
Conformational changes of Spo0F along the phosphotransfer pathway.
|
| |
J Bacteriol,
187,
8221-8227.
|
|
|
|
|
|
|
K.J.Hellingwerf
(2005).
Bacterial observations: a rudimentary form of intelligence?
|
| |
Trends Microbiol,
13,
152-158.
|
|
|
|
|
|
The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
Where a reference describes a PDB structure, the PDB
code is
shown on the right.
|
|
Links
