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UniProt functional annotation for P22259 | ||
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| UniProt code: P22259. |
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| Organism: | |
Escherichia coli (strain K12). |
| Taxonomy: | |
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia. |
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| Function: | |
Involved in the gluconeogenesis. Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP) through direct phosphoryl transfer between the nucleoside triphosphate and OAA. {ECO:0000255|HAMAP-Rule:MF_00453, ECO:0000269|PubMed:1701430, ECO:0000269|PubMed:6986370, ECO:0000269|PubMed:8226637}. |
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| Catalytic activity: | |
Reaction=ATP + oxaloacetate = ADP + CO2 + phosphoenolpyruvate; Xref=Rhea:RHEA:18617, ChEBI:CHEBI:16452, ChEBI:CHEBI:16526, ChEBI:CHEBI:30616, ChEBI:CHEBI:58702, ChEBI:CHEBI:456216; EC=4.1.1.49; Evidence={ECO:0000255|HAMAP-Rule:MF_00453, ECO:0000269|PubMed:6986370}; |
| Cofactor: | |
Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000255|HAMAP-Rule:MF_00453, ECO:0000269|PubMed:1701430, ECO:0000269|PubMed:17475535, ECO:0000269|PubMed:9406547, ECO:0000269|Ref.20, ECO:0000269|Ref.21}; Note=Binds 1 Mn(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_00453, ECO:0000269|PubMed:1701430, ECO:0000269|PubMed:17475535, ECO:0000269|PubMed:9406547, ECO:0000269|Ref.20, ECO:0000269|Ref.21}; |
| Activity regulation: | |
Allosterically activated by calcium. It may represent the only case of a monomeric, allosteric enzyme. {ECO:0000269|PubMed:12837799, ECO:0000269|PubMed:1701430, ECO:0000269|PubMed:6986370, ECO:0000269|PubMed:8609605}. |
| Biophysicochemical properties: | |
Kinetic parameters: KM=3.8 uM for manganese (at 31 degrees Celsius and pH 7.5) {ECO:0000269|PubMed:17475535, ECO:0000269|PubMed:6986370}; KM=0.11 mM for calcium (at 31 degrees Celsius and pH 7.5) {ECO:0000269|PubMed:17475535, ECO:0000269|PubMed:6986370}; KM=0.31 mM for OAA (at 31 degrees Celsius and pH 7.5) {ECO:0000269|PubMed:17475535, ECO:0000269|PubMed:6986370}; KM=0.51 mM for OAA (at pH 7.5) {ECO:0000269|PubMed:17475535, ECO:0000269|PubMed:6986370}; KM=1.4 mM for magnesium (at 31 degrees Celsius and pH 7.5) {ECO:0000269|PubMed:17475535, ECO:0000269|PubMed:6986370}; KM=11 mM for PEP (at pH 7.5) {ECO:0000269|PubMed:17475535, ECO:0000269|PubMed:6986370}; |
| Pathway: | |
Carbohydrate biosynthesis; gluconeogenesis. {ECO:0000255|HAMAP-Rule:MF_00453}. |
| Subunit: | |
Monomer. {ECO:0000255|HAMAP-Rule:MF_00453, ECO:0000269|PubMed:11724534, ECO:0000269|PubMed:12837799, ECO:0000269|PubMed:1701430, ECO:0000269|PubMed:17475535, ECO:0000269|PubMed:6986370, ECO:0000269|PubMed:8599762, ECO:0000269|PubMed:9406547, ECO:0000269|Ref.20, ECO:0000269|Ref.21}. |
| Subcellular location: | |
Cytoplasm. |
| Induction: | |
Induced upon entry into stationary phase and by cyclic AMP (cAMP). Repressed by glucose (catabolite repression) and by CsrA. {ECO:0000269|PubMed:6434512, ECO:0000269|PubMed:8393005}. |
| Mass spectrometry: | |
Mass=59656; Method=Electrospray; Evidence={ECO:0000269|PubMed:8609605}; |
| Similarity: | |
Belongs to the phosphoenolpyruvate carboxykinase (ATP) family. {ECO:0000255|HAMAP-Rule:MF_00453}. |
| Sequence caution: | |
Sequence=AAA24301.1; Type=Frameshift; Note=Translation N-terminally extended.; Evidence={ECO:0000305}; |
Annotations taken from UniProtKB at the EBI.