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PDBsum entry 1jwi
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protein Protein-protein interface(s) links
Toxin PDB id
1jwi

 

 

 

 

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Contents
Protein chains
122 a.a. *
123 a.a. *
Waters ×143
* Residue conservation analysis
PDB id:
1jwi
Name: Toxin
Title: Crystal structure of bitiscetin, a von willeband factor-dependent platelet aggregation inducer.
Structure: Bitiscetin. Chain: a. Synonym: bitiscetin alpha chain. Platelet aggregation inducer. Chain: b. Synonym: bitiscetin beta chain
Source: Bitis arietans. Puff adder. Organism_taxid: 8692. Secretion: venom. Secretion: venom
Biol. unit: Dimer (from PQS)
Resolution:
2.00Å     R-factor:   0.207     R-free:   0.252
Authors: S.Hirotsu,H.Mizuno,K.Fukuda,M.C.Qi,T.Matsui,J.Hamako,T.Morita, K.Titani
Key ref:
S.Hirotsu et al. (2001). Crystal structure of bitiscetin, a von Willebrand factor-dependent platelet aggregation inducer. Biochemistry, 40, 13592-13597. PubMed id: 11695907 DOI: 10.1021/bi0114933
Date:
04-Sep-01     Release date:   28-Nov-01    
PROCHECK
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 Headers
 References

Protein chain
Pfam   ArchSchema ?
Q7LZK5  (SLA_BITAR) -  Snaclec bitiscetin subunit alpha from Bitis arietans
Seq:
Struc: 		131 a.a.
122 a.a.
Protein chain
Pfam   ArchSchema ?
Q7LZK8  (SLB_BITAR) -  Snaclec bitiscetin subunit beta from Bitis arietans
Seq:
Struc: 		125 a.a.
123 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 

 
DOI no: 10.1021/bi0114933 Biochemistry 40:13592-13597 (2001)
PubMed id: 11695907  
 
 
Crystal structure of bitiscetin, a von Willebrand factor-dependent platelet aggregation inducer.
S.Hirotsu, H.Mizuno, K.Fukuda, M.C.Qi, T.Matsui, J.Hamako, T.Morita, K.Titani.
 
  ABSTRACT  
 
Bitiscetin, a C-type lectin-like protein isolated from the venom of the snake Bitis arientans, promotes the interactions between plasma von Willebrand factor (VWF) and platelet membrane glycoprotein Ib (GPIb) to induce platelet aggregation. We report here the crystal structure of bitiscetin at 2.0 A resolution. The overall fold is similar to those of coagulation factor IX/X-binding protein (IX/X-bp) and flavocetin-A (a GPIb-binding protein), although these three proteins are functionally distinct from one another. The characteristic property determining target recognition is explained mainly by the differences in the surface potential on the central concave surface. A negatively charged patch on the surface of bitiscetin is a candidate for the site of binding to the positively charged surface of the VWF A1 domain, as shown in the case of another platelet aggregation inducer, botrocetin. However, a positively charged patch near the central concave surface is unique for bitiscetin and suggests that it is the binding site for the negatively charged surface of the VWF A3 domain. Thus, the interactions accounting for VWF activation by bitiscetin possibly involve both the A1 and A3 domains of VWF, indicating a specific mechanism of VWF activation by bitiscetin.
 

Literature references that cite this PDB file's key reference

  PubMed id Reference
21256857 Z.Chen, J.Wu, Y.Zhang, X.Yang, G.Yu, S.Zhu, W.Lee, Q.Lu, and Y.Zhang (2011).
A novel platelet glycoprotein Ib-binding protein with human platelet aggregation-inhibiting activity from Trimeresurus jerdonii venom.
  Toxicon, 57, 672-679.  
18583525 A.A.Watson, J.A.Eble, and C.A.O'Callaghan (2008).
Crystal structure of rhodocytin, a ligand for the platelet-activating receptor CLEC-2.
  Protein Sci, 17, 1611-1616.
PDB code: 2vrp
18597489 E.Hooley, E.Papagrigoriou, A.Navdaev, A.V.Pandey, J.M.Clemetson, K.J.Clemetson, and J.Emsley (2008).
The crystal structure of the platelet activator aggretin reveals a novel (alphabeta)2 dimeric structure.
  Biochemistry, 47, 7831-7837.
PDB code: 3bx4
16336259 A.N.Zelensky, and J.E.Gready (2005).
The C-type lectin-like domain superfamily.
  FEBS J, 272, 6179-6217.  
15502319 G.Xu, M.Teng, L.Niu, P.Liu, Y.Dong, Q.Liu, Q.Huang, and Q.Hao (2004).
Purification, characterization, crystallization and preliminary X-ray crystallographic analysis of two novel C-type lectin-like proteins: Aall-A and Aall-B from Deinagkistrodon acutus venom.
  Acta Crystallogr D Biol Crystallogr, 60, 2035-2037.  
14684891 T.Batuwangala, M.Leduc, J.M.Gibbins, C.Bon, and E.Y.Jones (2004).
Structure of the snake-venom toxin convulxin.
  Acta Crystallogr D Biol Crystallogr, 60, 46-53.
PDB code: 1uos
12866057 A.N.Zelensky, and J.E.Gready (2003).
Comparative analysis of structural properties of the C-type-lectin-like domain (CTLD).
  Proteins, 52, 466-477.  
  12750409 H.Wu, A.Kuzmenko, S.Wan, L.Schaffer, A.Weiss, J.H.Fisher, K.S.Kim, and F.X.McCormack (2003).
Surfactant proteins A and D inhibit the growth of Gram-negative bacteria by increasing membrane permeability.
  J Clin Invest, 111, 1589-1602.  
14580195 K.Horii, D.Okuda, T.Morita, and H.Mizuno (2003).
Structural characterization of EMS16, an antagonist of collagen receptor (GPIa/IIa) from the venom of Echis multisquamatus.
  Biochemistry, 42, 12497-12502.
PDB code: 1ukm
12851390 N.Maita, K.Nishio, E.Nishimoto, T.Matsui, Y.Shikamoto, T.Morita, J.E.Sadler, and H.Mizuno (2003).
Crystal structure of von Willebrand factor A1 domain complexed with snake venom, bitiscetin: insight into glycoprotein Ibalpha binding mechanism induced by snake venom proteins.
  J Biol Chem, 278, 37777-37781.
PDB code: 1uex
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.

 

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