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UniProt functional annotation for A2RJT9 | ||
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| UniProt codes: A2RJT9, P54321. |
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| Organism: | |
Lactococcus lactis subsp. cremoris (strain MG1363). |
| Taxonomy: | |
Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae; Lactococcus. |
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| Function: | |
Catalyzes the conversion of dihydroorotate to orotate with fumarate as the electron acceptor. Molecular oxygen can replace fumarate in vitro, but cannot use NAD(+) as an electron acceptor. {ECO:0000269|PubMed:8021180}. |
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| Catalytic activity: | |
Reaction=(S)-dihydroorotate + fumarate = orotate + succinate; Xref=Rhea:RHEA:30059, ChEBI:CHEBI:29806, ChEBI:CHEBI:30031, ChEBI:CHEBI:30839, ChEBI:CHEBI:30864; EC=1.3.98.1; Evidence={ECO:0000269|PubMed:8021180}; |
| Cofactor: | |
Name=FMN; Xref=ChEBI:CHEBI:58210; Evidence={ECO:0000269|PubMed:9032071, ECO:0000269|PubMed:9655329}; Note=Binds 1 FMN per subunit. {ECO:0000269|PubMed:9032071, ECO:0000269|PubMed:9655329}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=18 uM for dihydroorotate {ECO:0000269|PubMed:12732650}; KM=250 uM for fumarate {ECO:0000269|PubMed:12732650}; Vmax=18.6 umol/min/mg enzyme {ECO:0000269|PubMed:12732650}; |
| Pathway: | |
Pyrimidine metabolism; UMP biosynthesis via de novo pathway. |
| Subunit: | |
Homodimer. {ECO:0000269|PubMed:9032071, ECO:0000269|PubMed:9655329}. |
| Subcellular location: | |
Cytoplasm {ECO:0000250}. |
| Similarity: | |
Belongs to the dihydroorotate dehydrogenase family. Type 1 subfamily. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.