UniProt functional annotation for Q05603



	UniProt functional annotation for Q05603

UniProt code: Q05603.

Organism: Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).

Taxonomy: Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Salmonella.

Function: Catalyzes the synthesis of alpha-ribazole-5'-phosphate from nicotinate mononucleotide (NAMN) and 5,6-dimethylbenzimidazole (DMB) (PubMed:8206834, PubMed:10587435, PubMed:11441022, Ref.7, PubMed:24121107). Able to use a variety of other nucleotide bases as substrate to create alternative lower ligands for cobamide (PubMed:11441022, Ref.7, PubMed:24121107). {ECO:0000269|PubMed:10587435, ECO:0000269|PubMed:11441022, ECO:0000269|PubMed:24121107, ECO:0000269|PubMed:8206834, ECO:0000269|Ref.7}.

Catalytic activity: Reaction=5,6-dimethylbenzimidazole + nicotinate beta-D-ribonucleotide = alpha-ribazole 5'-phosphate + H(+) + nicotinate; Xref=Rhea:RHEA:11196, ChEBI:CHEBI:15378, ChEBI:CHEBI:15890, ChEBI:CHEBI:32544, ChEBI:CHEBI:57502, ChEBI:CHEBI:57918; EC=2.4.2.21; Evidence={ECO:0000269|PubMed:8206834};

Pathway: Nucleoside biosynthesis; alpha-ribazole biosynthesis; alpha- ribazole from 5,6-dimethylbenzimidazole: step 1/2.

Subunit: Homodimer. {ECO:0000269|PubMed:10587435, ECO:0000269|PubMed:24121107, ECO:0000269|Ref.7}.

Disruption phenotype: No growth on cobinamide (CBI); double cobB-cobT deletion mutants do not grow on CBI and DMB (PubMed:8206834). {ECO:0000269|PubMed:8206834}.

Miscellaneous: In vitro assays of this enzyme are performed at pH 10 because at physiological pH (7.0) its activity is undetectable; this makes it difficult to assess the role of mutagenesis of potential proton acceptor active site residues (Glu-174 and Glu-317) (PubMed:24121107). {ECO:0000305|PubMed:24121107}.

Similarity: Belongs to the CobT family. {ECO:0000305}.

Sequence caution: Sequence=AAA27271.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305}; Sequence=AAA69297.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};

Annotations taken from UniProtKB at the EBI.


Organism:		Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
Taxonomy:		Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Salmonella.



Function:		Catalyzes the synthesis of alpha-ribazole-5'-phosphate from nicotinate mononucleotide (NAMN) and 5,6-dimethylbenzimidazole (DMB) (PubMed:8206834, PubMed:10587435, PubMed:11441022, Ref.7, PubMed:24121107). Able to use a variety of other nucleotide bases as substrate to create alternative lower ligands for cobamide (PubMed:11441022, Ref.7, PubMed:24121107). {ECO:0000269\|PubMed:10587435, ECO:0000269\|PubMed:11441022, ECO:0000269\|PubMed:24121107, ECO:0000269\|PubMed:8206834, ECO:0000269\|Ref.7}.


Catalytic activity:		Reaction=5,6-dimethylbenzimidazole + nicotinate beta-D-ribonucleotide = alpha-ribazole 5'-phosphate + H(+) + nicotinate; Xref=Rhea:RHEA:11196, ChEBI:CHEBI:15378, ChEBI:CHEBI:15890, ChEBI:CHEBI:32544, ChEBI:CHEBI:57502, ChEBI:CHEBI:57918; EC=2.4.2.21; Evidence={ECO:0000269\|PubMed:8206834};
Pathway:		Nucleoside biosynthesis; alpha-ribazole biosynthesis; alpha- ribazole from 5,6-dimethylbenzimidazole: step 1/2.
Subunit:		Homodimer. {ECO:0000269\|PubMed:10587435, ECO:0000269\|PubMed:24121107, ECO:0000269\|Ref.7}.
Disruption phenotype:		No growth on cobinamide (CBI); double cobB-cobT deletion mutants do not grow on CBI and DMB (PubMed:8206834). {ECO:0000269\|PubMed:8206834}.
Miscellaneous:		In vitro assays of this enzyme are performed at pH 10 because at physiological pH (7.0) its activity is undetectable; this makes it difficult to assess the role of mutagenesis of potential proton acceptor active site residues (Glu-174 and Glu-317) (PubMed:24121107). {ECO:0000305\|PubMed:24121107}.
Similarity:		Belongs to the CobT family. {ECO:0000305}.
Sequence caution:		Sequence=AAA27271.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305}; Sequence=AAA69297.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};