|
|
||
|
|
|
|
|
UniProt functional annotation for P16444 | ||
|
|
|
|
|
|
||
| UniProt code: P16444. |
|
||
| Organism: | |
Homo sapiens (Human). |
| Taxonomy: | |
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. |
|
||
|
||
| Function: | |
Hydrolyzes a wide range of dipeptides including the conversion of leukotriene D4 to leukotriene E4 (PubMed:2303490, PubMed:6334084, PubMed:31442408). Hydrolyzes cystinyl-bis-glycine (cys- bis-gly) formed during glutathione degradation (By similarity). Possesses also beta lactamase activity and hydrolytically inactivates beta-lactam antibiotics (PubMed:6334084). {ECO:0000250|UniProtKB:P31428, ECO:0000269|PubMed:2303490, ECO:0000269|PubMed:31442408, ECO:0000269|PubMed:6334084}. |
|
||
|
||
| Function: | |
Independently of its dipeptidase activity acts as an adhesion receptor for neutrophils recruitement from bloodstream into inflammed lungs and liver. {ECO:0000250|UniProtKB:P31428}. |
|
||
| Catalytic activity: | |
Reaction=an L-aminoacyl-L-amino acid + H2O = 2 an L-alpha-amino acid; Xref=Rhea:RHEA:48940, ChEBI:CHEBI:15377, ChEBI:CHEBI:59869, ChEBI:CHEBI:77460; EC=3.4.13.19; Evidence={ECO:0000255|PROSITE- ProRule:PRU10073, ECO:0000269|PubMed:2303490}; |
| Catalytic activity: | |
Reaction=H2O + leukotriene D4 = glycine + leukotriene E4; Xref=Rhea:RHEA:48616, ChEBI:CHEBI:15377, ChEBI:CHEBI:57305, ChEBI:CHEBI:57462, ChEBI:CHEBI:63166; Evidence={ECO:0000269|PubMed:2303490}; |
| Catalytic activity: | |
Reaction=a beta-lactam + H2O = a substituted beta-amino acid; Xref=Rhea:RHEA:20401, ChEBI:CHEBI:15377, ChEBI:CHEBI:35627, ChEBI:CHEBI:140347; EC=3.5.2.6; Evidence={ECO:0000269|PubMed:6334084}; |
| Catalytic activity: | |
Reaction=2 H2O + L-cystine-bis-glycine = 2 glycine + L-cystine; Xref=Rhea:RHEA:60520, ChEBI:CHEBI:15377, ChEBI:CHEBI:35491, ChEBI:CHEBI:57305, ChEBI:CHEBI:143812; Evidence={ECO:0000250|UniProtKB:P31428}; |
| Catalytic activity: | |
Reaction=glycyldehydrophenylalanine + H2O = 2,3-didehydrophenylalanine + glycine; Xref=Rhea:RHEA:62704, ChEBI:CHEBI:15377, ChEBI:CHEBI:57305, ChEBI:CHEBI:145925, ChEBI:CHEBI:145926; Evidence={ECO:0000269|PubMed:2303490, ECO:0000269|PubMed:31442408, ECO:0000269|PubMed:6334084}; |
| Cofactor: | |
Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000255|PROSITE-ProRule:PRU10073, ECO:0000269|PubMed:12144777}; Note=Binds 2 Zn(2+) ion per monomer. {ECO:0000269|PubMed:12144777}; |
| Activity regulation: | |
Inhibited by L-penicillamine (PubMed:31442408). Beta-lactamase activity is inhibited by cilastatin (PubMed:6334084, PubMed:31442408). {ECO:0000269|PubMed:31442408, ECO:0000269|PubMed:6334084}. |
| Biophysicochemical properties: | |
Kinetic parameters: KM=10.9 uM for imipenem {ECO:0000269|PubMed:6334084}; Vmax=44.5 umol/min/mg enzyme with imipenem as substrate {ECO:0000269|PubMed:6334084}; |
| Subunit: | |
Homodimer; disulfide-linked. {ECO:0000255|PROSITE- ProRule:PRU10073, ECO:0000269|PubMed:12144777}. |
| Subcellular location: | |
Apical cell membrane {ECO:0000269|PubMed:28413640}; Lipid-anchor, GPI-anchor {ECO:0000269|PubMed:2168407}. Cell projection, microvillus membrane {ECO:0000269|PubMed:2168407}; Lipid-anchor, GPI-anchor {ECO:0000269|PubMed:2168407}. Note=Brush border membrane. {ECO:0000250|UniProtKB:P31429}. |
| Tissue specificity: | |
Expressed in lung and kidneys. {ECO:0000269|PubMed:31442408, ECO:0000269|PubMed:8439558}. |
| Induction: | |
Up-regulated in n colorectal cancers. {ECO:0000269|PubMed:28413640}. |
| Similarity: | |
Belongs to the metallo-dependent hydrolases superfamily. Peptidase M19 family. {ECO:0000255|PROSITE-ProRule:PRU10073}. |
Annotations taken from UniProtKB at the EBI.