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PDBsum entry 1ipc
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RNA binding protein
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PDB id
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1ipc
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Contents |
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* Residue conservation analysis
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References listed in PDB file
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Key reference
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Title
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Crystal structures of 7-Methylguanosine 5'-Triphosphate (m(7)gtp)- And p(1)-7-Methylguanosine-P(3)-Adenosine-5',5'-Triphosphate (m(7)gpppa)-Bound human full-Length eukaryotic initiation factor 4e: biological importance of the c-Terminal flexible region.
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Authors
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K.Tomoo,
X.Shen,
K.Okabe,
Y.Nozoe,
S.Fukuhara,
S.Morino,
T.Ishida,
T.Taniguchi,
H.Hasegawa,
A.Terashima,
M.Sasaki,
Y.Katsuya,
K.Kitamura,
H.Miyoshi,
M.Ishikawa,
K.Miura.
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Ref.
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Biochem J, 2002,
362,
539-544.
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PubMed id
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Abstract
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The crystal structures of the full-length human eukaryotic initiation factor
(eIF) 4E complexed with two mRNA cap analogues [7-methylguanosine
5'-triphosphate (m(7)GTP) and
P(1)-7-methylguanosine-P(3)-adenosine-5',5'-triphosphate (m(7)GpppA)] were
determined at 2.0 A resolution (where 1 A=0.1 nm). The flexibility of the
C-terminal loop region of eIF4E complexed with m(7)GTP was significantly reduced
when complexed with m(7)GpppA, suggesting the importance of the second
nucleotide in the mRNA cap structure for the biological function of eIF4E,
especially the fixation and orientation of the C-terminal loop region, including
the eIF4E phosphorylation residue. The present results provide the structural
basis for the biological function of both N- and C-terminal mobile regions of
eIF4E in translation initiation, especially the regulatory function through the
switch-on/off of eIF4E-binding protein-eIF4E phosphorylation.
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