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PDBsum entry 1ibr
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protein ligands metals Protein-protein interface(s) links
Cell cycle,translation PDB id
1ibr

 

 

 

 

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Contents
Protein chains
169 a.a. *
458 a.a. *
Ligands
GNP ×2
Metals
_MG ×2
Waters ×312
* Residue conservation analysis
PDB id:
1ibr
Name: Cell cycle,translation
Title: Complex of ran with importin beta
Structure: Gtp-binding nuclear protein ran. Chain: a, c. Synonym: ran. Tc4. Ran gtpase. Androgen receptor-associated protein 24. Engineered: yes. Importin beta-1 subunit. Chain: b, d. Fragment: residues 1-462. Synonym: karyopherin beta-1 subunit. P95. Nuclear factor p97.
Source: Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Expression_system_variant: de3. Organism_taxid: 9606
Biol. unit: Dimer (from PQS)
Resolution:
2.30Å     R-factor:   0.245     R-free:   0.272
Authors: I.R.Vetter,A.Arndt,U.Kutay,D.Goerlich,A.Wittinghofer
Key ref:
I.R.Vetter et al. (1999). Structural view of the Ran-Importin beta interaction at 2.3 A resolution. Cell, 97, 635-646. PubMed id: 10367892 DOI: 10.1016/S0092-8674(00)80774-6
Date:
14-May-99     Release date:   03-Jun-99    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chains
Pfam   ArchSchema ?
P62826  (RAN_HUMAN) -  GTP-binding nuclear protein Ran from Homo sapiens
Seq:
Struc: 		216 a.a.
169 a.a.
Protein chains
Pfam   ArchSchema ?
Q14974  (IMB1_HUMAN) -  Importin subunit beta-1 from Homo sapiens
Seq:
Struc: 		 
Seq:
Struc: 		876 a.a.
458 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Enzyme reactions 
   Enzyme class: Chains A, C: E.C.3.6.5.-  - ?????
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]

 

 
DOI no: 10.1016/S0092-8674(00)80774-6 Cell 97:635-646 (1999)
PubMed id: 10367892  
 
 
Structural view of the Ran-Importin beta interaction at 2.3 A resolution.
I.R.Vetter, A.Arndt, U.Kutay, D.Görlich, A.Wittinghofer.
 
  ABSTRACT  
 
Transport receptors of the Importin beta family shuttle between the nucleus and cytoplasm and mediate transport of macromolecules through nuclear pore complexes. They interact specifically with the GTP-binding protein Ran, which in turn regulates their interaction with cargo. Here, we report the three-dimensional structure of a complex between Ran bound to the nonhydrolyzable GTP analog GppNHp and a 462-residue fragment from Importin beta. The structure of Importin beta shows 10 tandem repeats resembling HEAT and Armadillo motifs. They form an irregular crescent, the concave site of which forms the interface with Ran-triphosphate. The importin-binding site of Ran does not overlap with that of the Ran-binding domain of RanBP2.
 
  Selected figure(s)  
 
Figure 4.
Figure 4. Structure of the Complex(A) Schematic representation of the structure of the complex, indicating the contacts of residues on Impβ with those of Ran. Interactions found in only one of the two molecules have an additional A,B identifier on the residue number. Open circles in Impβ[N] indicate the position of the B helices of the repeats with their numbers in red.(B) Ribbon representation of the complex with Ran in red, Impβ in green, superimposed with RanGDP in blue to highlight the potential clashes in switch I and the C-terminal end. GppNHp and Mg^2+ are shown as black ball-and-stick models.(C) Electrostatic surface potential of Impβ and RanGppNHp in and close to the interface (GRASP; [53]). The 180° rotation of Ran is indicated.
Figure 5.
Figure 5. Flexibility of ImpβB factor representation of the two Impβ molecules in the asymmetric unit of the crystal with a color code, where red indicates high B factors and blue indicates low B factors. This indicates the different flexibilities in the two molecules, both inside and outside the Ran interface.
 
  The above figures are reprinted by permission from Cell Press: Cell (1999, 97, 635-646) copyright 1999.  
  Figures were selected by an automated process.  

Literature references that cite this PDB file's key reference

  PubMed id Reference
21481778 E.Karaca, and A.M.Bonvin (2011).
A multidomain flexible docking approach to deal with large conformational changes in the modeling of biomolecular complexes.
  Structure, 19, 555-565.  
21145229 S.J.Lee, C.Jiko, E.Yamashita, and T.Tsukihara (2011).
Selective nuclear export mechanism of small RNAs.
  Curr Opin Struct Biol, 21, 101-108.  
20547757 C.Liu, M.Takahashi, Y.Li, T.J.Dillon, S.Kaech, and P.J.Stork (2010).
The interaction of Epac1 and Ran promotes Rap1 activation at the nuclear envelope.
  Mol Cell Biol, 30, 3956-3969.  
20927102 E.H.Rubinson, A.S.Gowda, T.E.Spratt, B.Gold, and B.F.Eichman (2010).
An unprecedented nucleic acid capture mechanism for excision of DNA damage.
  Nature, 468, 406-411.
PDB codes: 3jx7 3jxy 3jxz 3jy1
20826343 J.K.Forwood, A.Lange, U.Zachariae, M.Marfori, C.Preast, H.Grubmüller, M.Stewart, A.H.Corbett, and B.Kobe (2010).
Quantitative structural analysis of importin-β flexibility: paradigm for solenoid protein structures.
  Structure, 18, 1171-1183.
PDB code: 3nd2
20421988 L.J.Colwell, M.P.Brenner, and K.Ribbeck (2010).
Charge as a selection criterion for translocation through the nuclear pore complex.
  PLoS Comput Biol, 6, e1000747.  
19435463 S.Huang, I.S.Chang, W.Lin, W.Ye, R.Z.Luo, Z.Lu, Y.Lu, K.Zhang, W.S.Liao, T.Tao, R.C.Bast, X.Chen, and Y.Yu (2010).
ARHI (DIRAS3), an imprinted tumour suppressor gene, binds to importins and blocks nuclear import of cargo proteins.
  Biosci Rep, 30, 159-168.  
19680239 A.G.Cook, N.Fukuhara, M.Jinek, and E.Conti (2009).
Structures of the tRNA export factor in the nuclear and cytosolic states.
  Nature, 461, 60-65.
PDB codes: 3ibv 3icq
19761543 C.Ptak, A.M.Anderson, R.J.Scott, D.Van de Vosse, R.S.Rogers, Y.Sydorskyy, J.D.Aitchison, and R.W.Wozniak (2009).
A role for the karyopherin Kap123p in microtubule stability.
  Traffic, 10, 1619-1634.  
19680228 D.Mohr, S.Frey, T.Fischer, T.Güttler, and D.Görlich (2009).
Characterisation of the passive permeability barrier of nuclear pore complexes.
  EMBO J, 28, 2541-2553.  
19124778 J.C.Phillips (2009).
Scaling and self-organized criticality in proteins II.
  Proc Natl Acad Sci U S A, 106, 3113-3118.  
18984568 K.F.Pulliam, M.B.Fasken, L.M.McLane, J.V.Pulliam, and A.H.Corbett (2009).
The Classical Nuclear Localization Signal Receptor, Importin-{alpha}, Is Required for Efficient Transition Through the G1/S Stage of the Cell Cycle in Saccharomyces cerevisiae.
  Genetics, 181, 105-118.  
19577735 S.A.Adam (2009).
The nuclear transport machinery in Caenorhabditis elegans: A central role in morphogenesis.
  Semin Cell Dev Biol, 20, 576-581.  
19389996 T.Monecke, T.Güttler, P.Neumann, A.Dickmanns, D.Görlich, and R.Ficner (2009).
Crystal structure of the nuclear export receptor CRM1 in complex with Snurportin1 and RanGTP.
  Science, 324, 1087-1091.
PDB code: 3gjx
19368893 T.Zhang, S.Li, Y.Zhang, C.Zhong, Z.Lai, and J.Ding (2009).
Crystal structure of the ARL2-GTP-BART complex reveals a novel recognition and binding mode of small GTPase with effector.
  Structure, 17, 602-610.
PDB codes: 3doe 3dof
17729269 A.May, and M.Zacharias (2008).
Energy minimization in low-frequency normal modes to efficiently allow for global flexibility during systematic protein-protein docking.
  Proteins, 70, 794-809.  
18946488 D.Komander, R.Garg, P.T.Wan, A.J.Ridley, and D.Barford (2008).
Mechanism of multi-site phosphorylation from a ROCK-I:RhoE complex structure.
  EMBO J, 27, 3175-3185.
PDB code: 2v55
18187419 G.Mitrousis, A.S.Olia, N.Walker-Kopp, and G.Cingolani (2008).
Molecular basis for the recognition of snurportin 1 by importin beta.
  J Biol Chem, 283, 7877-7884.
PDB codes: 2p8q 2q5d
18611384 N.Schrader, C.Koerner, K.Koessmeier, J.A.Bangert, A.Wittinghofer, R.Stoll, and I.R.Vetter (2008).
The crystal structure of the Ran-Nup153ZnF2 complex: a general Ran docking site at the nuclear pore complex.
  Structure, 16, 1116-1125.
PDB codes: 2k0c 3ch5
18206968 N.Schrader, P.Stelter, D.Flemming, R.Kunze, E.Hurt, and I.R.Vetter (2008).
Structural basis of the nic96 subcomplex organization in the nuclear pore channel.
  Mol Cell, 29, 46-55.
PDB code: 2rfo
18478030 P.R.Clarke, and C.Zhang (2008).
Spatial and temporal coordination of mitosis by Ran GTPase.
  Nat Rev Mol Cell Biol, 9, 464-477.  
18845677 S.Otsuka, S.Iwasaka, Y.Yoneda, K.Takeyasu, and S.H.Yoshimura (2008).
Individual binding pockets of importin-beta for FG-nucleoporins have different binding properties and different sensitivities to RanGTP.
  Proc Natl Acad Sci U S A, 105, 16101-16106.  
18547523 U.Zachariae, and H.Grubmüller (2008).
Importin-beta: structural and dynamic determinants of a molecular spring.
  Structure, 16, 906-915.  
18366719 V.A.Delmar, R.C.Chan, and D.J.Forbes (2008).
Xenopus importin beta validates human importin beta as a cell cycle negative regulator.
  BMC Cell Biol, 9, 14.  
17506639 A.Cook, F.Bono, M.Jinek, and E.Conti (2007).
Structural biology of nucleocytoplasmic transport.
  Annu Rev Biochem, 76, 647-671.  
17548362 A.S.Philips, J.C.Kwok, and B.H.Chong (2007).
Analysis of the signals and mechanisms mediating nuclear trafficking of GATA-4. Loss of DNA binding is associated with localization in intranuclear speckles.
  J Biol Chem, 282, 24915-24927.  
18073111 G.Buhrman, G.Wink, and C.Mattos (2007).
Transformation efficiency of RasQ61 mutants linked to structural features of the switch regions in the presence of Raf.
  Structure, 15, 1618-1629.
PDB codes: 2rga 2rgb 2rgc 2rgd 2rge 2rgg
17426026 M.M.Higa, S.L.Alam, W.I.Sundquist, and K.S.Ullman (2007).
Molecular characterization of the Ran-binding zinc finger domain of Nup153.
  J Biol Chem, 282, 17090-17100.
PDB code: 2gqe
17287812 M.Stewart (2007).
Molecular mechanism of the nuclear protein import cycle.
  Nat Rev Mol Cell Biol, 8, 195-208.  
17229728 T.Morita, A.Yamashita, I.Kashima, K.Ogata, S.Ishiura, and S.Ohno (2007).
Distant N- and C-terminal domains are required for intrinsic kinase activity of SMG-1, a critical component of nonsense-mediated mRNA decay.
  J Biol Chem, 282, 7799-7808.  
17215520 X.Liu, and X.F.Zheng (2007).
Endoplasmic reticulum and Golgi localization sequences for mammalian target of rapamycin.
  Mol Biol Cell, 18, 1073-1082.  
16421734 A.S.Madrid, and K.Weis (2006).
Nuclear transport is becoming crystal clear.
  Chromosoma, 115, 98.  
16901787 B.J.Lee, A.E.Cansizoglu, K.E.Süel, T.H.Louis, Z.Zhang, and Y.M.Chook (2006).
Rules for nuclear localization sequence recognition by karyopherin beta 2.
  Cell, 126, 543-558.
PDB code: 2h4m
16567089 E.Conti, C.W.Müller, and M.Stewart (2006).
Karyopherin flexibility in nucleocytoplasmic transport.
  Curr Opin Struct Biol, 16, 237-244.  
16689644 H.Stark, and R.Lührmann (2006).
Cryo-electron microscopy of spliceosomal components.
  Annu Rev Biophys Biomol Struct, 35, 435-457.  
16240320 H.Yagisawa (2006).
Nucleocytoplasmic shuttling of phospholipase C-delta1: a link to Ca2+.
  J Cell Biochem, 97, 233-243.  
16407315 M.Arnold, A.Nath, D.Wohlwend, and R.H.Kehlenbach (2006).
Transportin is a major nuclear import receptor for c-Fos: a novel mode of cargo interaction.
  J Biol Chem, 281, 5492-5499.  
16819590 O.Julien, I.Gignac, A.Hutton, A.Yee, C.H.Arrowsmith, and S.M.Gagné (2006).
MTH187 from Methanobacterium thermoautotrophicum has three HEAT-like repeats.
  J Biomol NMR, 35, 149-154.
PDB code: 1te4
17215878 R.Truant, R.Atwal, and A.Burtnik (2006).
Hypothesis: Huntingtin may function in membrane association and vesicular trafficking.
  Biochem Cell Biol, 84, 912-917.  
  16880557 T.Imasaki, T.Shimizu, H.Hashimoto, Y.Hidaka, M.Yamada, and M.Sato (2006).
Crystallization and preliminary X-ray crystallographic studies of transportin 1 in complex with nucleocytoplasmic shuttling and nuclear localization fragments.
  Acta Crystallogr Sect F Struct Biol Cryst Commun, 62, 785-787.  
15880121 H.Viadiu, O.Stemmann, M.W.Kirschner, and T.Walz (2005).
Domain structure of separase and its binding to securin as determined by EM.
  Nat Struct Mol Biol, 12, 552-553.  
15702987 L.F.Pemberton, and B.M.Paschal (2005).
Mechanisms of receptor-mediated nuclear import and nuclear export.
  Traffic, 6, 187-198.  
15611084 M.H.Chen, I.Ben-Efraim, G.Mitrousis, N.Walker-Kopp, P.J.Sims, and G.Cingolani (2005).
Phospholipid scramblase 1 contains a nonclassical nuclear localization signal with unique binding site in importin alpha.
  J Biol Chem, 280, 10599-10606.
PDB code: 1y2a
15780942 M.M.Golas, B.Sander, C.L.Will, R.Lührmann, and H.Stark (2005).
Major conformational change in the complex SF3b upon integration into the spliceosomal U11/U12 di-snRNP as revealed by electron cryomicroscopy.
  Mol Cell, 17, 869-883.  
15778719 M.Schmidt, W.Haas, B.Crosas, P.G.Santamaria, S.P.Gygi, T.Walz, and D.Finley (2005).
The HEAT repeat protein Blm10 regulates the yeast proteasome by capping the core particle.
  Nat Struct Mol Biol, 12, 294-303.  
15726638 P.B.Crowley, and A.Golovin (2005).
Cation-pi interactions in protein-protein interfaces.
  Proteins, 59, 231-239.  
15864299 R.Nevo, V.Brumfeld, R.Kapon, P.Hinterdorfer, and Z.Reich (2005).
Direct measurement of protein energy landscape roughness.
  EMBO Rep, 6, 482-486.  
15864302 S.J.Lee, Y.Matsuura, S.M.Liu, and M.Stewart (2005).
Structural basis for nuclear import complex dissociation by RanGTP.
  Nature, 435, 693-696.
PDB code: 2bku
16338415 T.A.Isgro, and K.Schulten (2005).
Binding dynamics of isolated nucleoporin repeat regions to importin-beta.
  Structure, 13, 1869-1879.  
15882446 Y.H.Saitoh, K.Ogawa, and T.Nishimoto (2005).
Brl1p -- a novel nuclear envelope protein required for nuclear transport.
  Traffic, 6, 502-517.  
16222336 Y.Matsuura, and M.Stewart (2005).
Nup50/Npap60 function in nuclear protein import complex disassembly and importin recycling.
  EMBO J, 24, 3681-3689.
PDB codes: 2c1m 2c1t
15525506 A.Harel, and D.J.Forbes (2004).
Importin beta: conducting a much larger cellular symphony.
  Mol Cell, 16, 319-330.  
14978301 C.Blouin, D.Butt, and A.J.Roger (2004).
Rapid evolution in conformational space: a study of loop regions in a ubiquitous GTP binding domain.
  Protein Sci, 13, 608-616.  
14730349 C.C.Milburn, J.Boudeau, M.Deak, D.R.Alessi, and D.M.van Aalten (2004).
Crystal structure of MO25 alpha in complex with the C terminus of the pseudo kinase STE20-related adaptor.
  Nat Struct Mol Biol, 11, 193-200.
PDB codes: 1upk 1upl
15574331 C.Petosa, G.Schoehn, P.Askjaer, U.Bauer, M.Moulin, U.Steuerwald, M.Soler-López, F.Baudin, I.W.Mattaj, and C.W.Müller (2004).
Architecture of CRM1/Exportin1 suggests how cooperativity is achieved during formation of a nuclear export complex.
  Mol Cell, 16, 761-775.
PDB code: 1w9c
15653425 E.J.Helmreich (2004).
Structural flexibility of small GTPases. Can it explain their functional versatility?
  Biol Chem, 385, 1121-1136.  
14991001 L.Xu, and J.Massagué (2004).
Nucleocytoplasmic shuttling of signal transducers.
  Nat Rev Mol Cell Biol, 5, 209-219.  
14970380 M.Dlakić, and D.Tollervey (2004).
The Noc proteins involved in ribosome synthesis and export contain divergent HEAT repeats.
  RNA, 10, 351-354.  
15247908 M.Lu, J.M.Kinchen, K.L.Rossman, C.Grimsley, C.deBakker, E.Brugnera, A.C.Tosello-Trampont, L.B.Haney, D.Klingele, J.Sondek, M.O.Hengartner, and K.S.Ravichandran (2004).
PH domain of ELMO functions in trans to regulate Rac activation via Dock180.
  Nat Struct Mol Biol, 11, 756-762.  
15096275 M.N.Offman, R.N.Nurtdinov, M.S.Gelfand, and D.Frishman (2004).
No statistical support for correlation between the positions of protein interaction sites and alternatively spliced regions.
  BMC Bioinformatics, 5, 41.  
14729571 M.Oeffinger, M.Dlakic, and D.Tollervey (2004).
A pre-ribosome-associated HEAT-repeat protein is required for export of both ribosomal subunits.
  Genes Dev, 18, 196-209.  
14561738 N.Fukuhara, E.Fernandez, J.Ebert, E.Conti, and D.Svergun (2004).
Conformational variability of nucleo-cytoplasmic transport factors.
  J Biol Chem, 279, 2176-2181.  
15454457 R.Nevo, V.Brumfeld, M.Elbaum, P.Hinterdorfer, and Z.Reich (2004).
Direct discrimination between models of protein activation by single-molecule force measurements.
  Biophys J, 87, 2630-2634.  
15602554 Y.Matsuura, and M.Stewart (2004).
Structural basis for the assembly of a nuclear export complex.
  Nature, 432, 872-877.
PDB code: 1wa5
12578832 A.Braunwarth, M.Fromont-Racine, P.Legrain, F.R.Bischoff, T.Gerstberger, E.Hurt, and M.Kunzler (2003).
Identification and characterization of a novel RanGTP-binding protein in the yeast Saccharomyces cerevisiae.
  J Biol Chem, 278, 15397-15405.  
12671004 A.F.Neuwald, N.Kannan, A.Poleksic, N.Hata, and J.S.Liu (2003).
Ran's C-terminal, basic patch, and nucleotide exchange mechanisms in light of a canonical structure for Rab, Rho, Ras, and Ran GTPases.
  Genome Res, 13, 673-692.  
12581669 C.Herrmann (2003).
Ras-effector interactions: after one decade.
  Curr Opin Struct Biol, 13, 122-129.  
12594203 C.Koerner, T.Guan, L.Gerace, and G.Cingolani (2003).
Synergy of silent and hot spot mutations in importin beta reveals a dynamic mechanism for recognition of a nuclear localization signal.
  J Biol Chem, 278, 16216-16221.  
12802078 C.Rollenhagen, P.Mühlhäusser, U.Kutay, and N.Panté (2003).
Importin beta-depending nuclear import pathways: role of the adapter proteins in the docking and releasing steps.
  Mol Biol Cell, 14, 2104-2115.  
14514689 D.Owen, P.N.Lowe, D.Nietlispach, C.E.Brosnan, D.Y.Chirgadze, P.J.Parker, T.L.Blundell, and H.R.Mott (2003).
Molecular dissection of the interaction between the small G proteins Rac1 and RhoA and protein kinase C-related kinase 1 (PRK1).
  J Biol Chem, 278, 50578-50587.
PDB code: 1urf
12490886 E.Conti (2003).
The hitchhiker's guide to the nucleus.
  Nat Struct Biol, 10, 8-9.  
12885761 J.Bednenko, G.Cingolani, and L.Gerace (2003).
Importin beta contains a COOH-terminal nucleoporin binding region important for nuclear transport.
  J Cell Biol, 162, 391-401.  
12600309 K.Weis (2003).
Regulating access to the genome: nucleocytoplasmic transport throughout the cell cycle.
  Cell, 112, 441-451.  
12889070 L.Smith, N.Van Hateren, J.Willan, R.Romero, G.Blanco, P.Siggers, J.Walsh, R.Banerjee, P.Denny, C.Ponting, and A.Greenfield (2003).
Candidate testis-determining gene, Maestro (Mro), encodes a novel HEAT repeat protein.
  Dev Dyn, 227, 600-607.  
12738865 M.M.Golas, B.Sander, C.L.Will, R.Lührmann, and H.Stark (2003).
Molecular architecture of the multiprotein splicing factor SF3b.
  Science, 300, 980-984.  
14645832 M.Stewart (2003).
Structural biology. Nuclear trafficking.
  Science, 302, 1513-1514.  
12672802 M.T.Harreman, P.E.Cohen, M.R.Hodel, G.J.Truscott, A.H.Corbett, and A.E.Hodel (2003).
Characterization of the auto-inhibitory sequence within the N-terminal domain of importin alpha.
  J Biol Chem, 278, 21361-21369.  
12808444 R.Nevo, C.Stroh, F.Kienberger, D.Kaftan, V.Brumfeld, M.Elbaum, Z.Reich, and P.Hinterdorfer (2003).
A molecular switch between alternative conformational states in the complex of Ran and importin beta1.
  Nat Struct Biol, 10, 553-557.  
12571241 R.P.Darst, A.Dasgupta, C.Zhu, J.Y.Hsu, A.Vroom, T.Muldrow, and D.T.Auble (2003).
Mot1 regulates the DNA binding activity of free TATA-binding protein in an ATP-dependent manner.
  J Biol Chem, 278, 13216-13226.  
12839989 S.Fukai, H.T.Matern, J.R.Jagath, R.H.Scheller, and A.T.Brunger (2003).
Structural basis of the interaction between RalA and Sec5, a subunit of the sec6/8 complex.
  EMBO J, 22, 3267-3278.
PDB code: 1uad
14501140 S.J.Lee, T.Sekimoto, E.Yamashita, E.Nagoshi, A.Nakagawa, H.Tanaka, Y.Yoneda, and T.Tsukihara (2003).
Crystallization and preliminary crystallographic analysis of the importin-beta-SREBP-2 complex.
  Acta Crystallogr D Biol Crystallogr, 59, 1866-1868.  
14645851 S.J.Lee, T.Sekimoto, E.Yamashita, E.Nagoshi, A.Nakagawa, N.Imamoto, M.Yoshimura, H.Sakai, K.T.Chong, T.Tsukihara, and Y.Yoneda (2003).
The structure of importin-beta bound to SREBP-2: nuclear import of a transcription factor.
  Science, 302, 1571-1575.
PDB code: 1ukl
12134081 E.Allemand, S.Dokudovskaya, R.Bordonné, and J.Tazi (2002).
A conserved Drosophila transportin-serine/arginine-rich (SR) protein permits nuclear import of Drosophila SR protein splicing factors and their antagonist repressor splicing factor 1.
  Mol Biol Cell, 13, 2436-2447.  
12504010 G.Cingolani, J.Bednenko, M.T.Gillespie, and L.Gerace (2002).
Molecular basis for the recognition of a nonclassical nuclear localization signal by importin beta.
  Mol Cell, 10, 1345-1353.
PDB code: 1m5n
12379151 H.Takano, and J.F.Gusella (2002).
The predominantly HEAT-like motif structure of huntingtin and its association and coincident nuclear entry with dorsal, an NF-kB/Rel/dorsal family transcription factor.
  BMC Neurosci, 3, 15.  
  12102729 J.E.Garbarino, and I.R.Gibbons (2002).
Expression and genomic analysis of midasin, a novel and highly conserved AAA protein distantly related to dynein.
  BMC Genomics, 3, 18.  
11815630 J.Huber, A.Dickmanns, and R.Lührmann (2002).
The importin-beta binding domain of snurportin1 is responsible for the Ran- and energy-independent nuclear import of spliceosomal U snRNPs in vitro.
  J Cell Biol, 156, 467-479.  
12376537 K.M.Jacques, Z.Nie, S.Stauffer, D.S.Hirsch, L.X.Chen, K.T.Stanley, and P.A.Randazzo (2002).
Arf1 dissociates from the clathrin adaptor GGA prior to being inactivated by Arf GTPase-activating proteins.
  J Biol Chem, 277, 47235-47241.  
12034733 K.Plafker, and I.G.Macara (2002).
Fluorescence resonance energy transfer biosensors that detect Ran conformational changes and a Ran x GDP-importin-beta -RanBP1 complex in vitro and in intact cells.
  J Biol Chem, 277, 30121-30127.  
11875068 L.Shu, X.Zhang, and P.J.Houghton (2002).
Myogenic differentiation is dependent on both the kinase function and the N-terminal sequence of mammalian target of rapamycin.
  J Biol Chem, 277, 16726-16732.  
12372823 R.Bayliss, T.Littlewood, L.A.Strawn, S.R.Wente, and M.Stewart (2002).
GLFG and FxFG nucleoporins bind to overlapping sites on importin-beta.
  J Biol Chem, 277, 50597-50606.
PDB codes: 1o6o 1o6p
12151228 S.Djordjevic, and P.C.Driscoll (2002).
Structural insight into substrate specificity and regulatory mechanisms of phosphoinositide 3-kinases.
  Trends Biochem Sci, 27, 426-432.  
11689570 Y.V.Budovskaya, H.Hama, D.B.DeWald, and P.K.Herman (2002).
The C terminus of the Vps34p phosphoinositide 3-kinase is necessary and sufficient for the interaction with the Vps15p protein kinase.
  J Biol Chem, 277, 287-294.  
  11423015 A.C.Ström, and K.Weis (2001).
Importin-beta-like nuclear transport receptors.
  Genome Biol, 2, REVIEWS3008.  
11229403 C.Wiese, A.Wilde, M.S.Moore, S.A.Adam, A.Merdes, and Y.Zheng (2001).
Role of importin-beta in coupling Ran to downstream targets in microtubule assembly.
  Science, 291, 653-656.  
11343901 E.Conti, and E.Izaurralde (2001).
Nucleocytoplasmic transport enters the atomic age.
  Curr Opin Cell Biol, 13, 310-319.  
11283257 E.Nagoshi, and Y.Yoneda (2001).
Dimerization of sterol regulatory element-binding protein 2 via the helix-loop-helix-leucine zipper domain is a prerequisite for its nuclear localization mediated by importin beta.
  Mol Cell Biol, 21, 2779-2789.  
11729264 I.G.Macara (2001).
Transport into and out of the nucleus.
  Microbiol Mol Biol Rev, 65, 570.  
11701921 I.R.Vetter, and A.Wittinghofer (2001).
The guanine nucleotide-binding switch in three dimensions.
  Science, 294, 1299-1304.  
11738594 K.D.Corbett, and T.Alber (2001).
The many faces of Ras: recognition of small GTP-binding proteins.
  Trends Biochem Sci, 26, 710-716.  
11571268 L.Englmeier, M.Fornerod, F.R.Bischoff, C.Petosa, I.W.Mattaj, and U.Kutay (2001).
RanBP3 influences interactions between CRM1 and its nuclear protein export substrates.
  EMBO Rep, 2, 926-932.  
11336674 L.Renault, J.Kuhlmann, A.Henkel, and A.Wittinghofer (2001).
Structural basis for guanine nucleotide exchange on Ran by the regulator of chromosome condensation (RCC1).
  Cell, 105, 245-255.
PDB code: 1i2m
  11251069 P.Maurer, M.Redd, J.Solsbacher, F.R.Bischoff, M.Greiner, A.V.Podtelejnikov, M.Mann, K.Stade, K.Weis, and G.Schlenstedt (2001).
The nuclear export receptor Xpo1p forms distinct complexes with NES transport substrates and the yeast Ran binding protein 1 (Yrb1p).
  Mol Biol Cell, 12, 539-549.  
11238901 R.Vongsamphanh, P.K.Fortier, and D.Ramotar (2001).
Pir1p mediates translocation of the yeast Apn1p endonuclease into the mitochondria to maintain genomic stability.
  Mol Cell Biol, 21, 1647-1655.  
11042144 A.F.Neuwald, and T.Hirano (2000).
HEAT repeats associated with condensins, cohesins, and other complexes involved in chromosome-related functions.
  Genome Res, 10, 1445-1452.  
10995230 C.I.Villa Braslavsky, C.Nowak, D.Görlich, A.Wittinghofer, and J.Kuhlmann (2000).
Different structural and kinetic requirements for the interaction of Ran with the Ran-binding domains from RanBP2 and importin-beta.
  Biochemistry, 39, 11629-11639.  
10660567 C.Petersen, N.Orem, J.Trueheart, J.W.Thorner, and I.G.Macara (2000).
Random mutagenesis and functional analysis of the Ran-binding protein, RanBP1.
  J Biol Chem, 275, 4081-4091.  
10747019 C.Zwahlen, S.C.Li, L.E.Kay, T.Pawson, and J.D.Forman-Kay (2000).
Multiple modes of peptide recognition by the PTB domain of the cell fate determinant Numb.
  EMBO J, 19, 1505-1515.
PDB code: 1ddm
10873829 D.J.Owen, and J.P.Luzio (2000).
Structural insights into clathrin-mediated endocytosis.
  Curr Opin Cell Biol, 12, 467-474.  
10967423 J.K.Hood, and P.A.Silver (2000).
Diverse nuclear transport pathways regulate cell proliferation and oncogenesis.
  Biochim Biophys Acta, 1471, M31-M41.  
11129790 N.Imamoto (2000).
Diversity in nucleocytoplasmic transport pathways.
  Cell Struct Funct, 25, 207-216.  
11208130 R.Bayliss, A.H.Corbett, and M.Stewart (2000).
The molecular mechanism of transport of macromolecules through nuclear pore complexes.
  Traffic, 1, 448-456.  
10929717 R.Bayliss, T.Littlewood, and M.Stewart (2000).
Structural basis for the interaction between FxFG nucleoporin repeats and importin-beta in nuclear trafficking.
  Cell, 102, 99.
PDB code: 1f59
10666614 R.P.Baker, and M.Stewart (2000).
Crystallization and preliminary X-ray diffraction analysis of the Saccharomyces cerevisiae ran-binding protein Mog1p.
  Acta Crystallogr D Biol Crystallogr, 56, 229-231.  
11137006 S.Panizza, T.Tanaka, A.Hochwagen, F.Eisenhaber, and K.Nasmyth (2000).
Pds5 cooperates with cohesin in maintaining sister chromatid cohesion.
  Curr Biol, 10, 1557-1564.  
11086011 U.Fleig, S.S.Salus, I.Karig, and S.Sazer (2000).
The fission yeast ran GTPase is required for microtubule integrity.
  J Cell Biol, 151, 1101-1111.  
  10888680 X.Pan, P.Roberts, Y.Chen, E.Kvam, N.Shulga, K.Huang, S.Lemmon, and D.S.Goldfarb (2000).
Nucleus-vacuole junctions in Saccharomyces cerevisiae are formed through the direct interaction of Vac8p with Nvj1p.
  Mol Biol Cell, 11, 2445-2457.  
10801459 Y.Azuma, and M.Dasso (2000).
The role of Ran in nuclear function.
  Curr Opin Cell Biol, 12, 302-307.  
10525531 A.A.Titov, and G.Blobel (1999).
The karyopherin Kap122p/Pdr6p imports both subunits of the transcription factor IIA into the nucleus.
  J Cell Biol, 147, 235-246.  
10611974 D.Görlich, and U.Kutay (1999).
Transport between the cell nucleus and the cytoplasm.
  Annu Rev Cell Dev Biol, 15, 607-660.  
10445030 L.M.Rice, and A.T.Brunger (1999).
Crystal structure of the vesicular transport protein Sec17: implications for SNAP function in SNARE complex disassembly.
  Mol Cell, 4, 85-95.
PDB code: 1qqe
10619026 R.Maesaki, K.Ihara, T.Shimizu, S.Kuroda, K.Kaibuchi, and T.Hakoshima (1999).
The structural basis of Rho effector recognition revealed by the crystal structure of human RhoA complexed with the effector domain of PKN/PRK1.
  Mol Cell, 4, 793-803.
PDB code: 1cxz
10508790 S.J.Gamblin, and S.J.Smerdon (1999).
Nuclear transport: what a kary-on!
  Structure, 7, R199-R204.  
10490335 Y.M.Chook, G.Cingolani, E.Conti, M.Stewart, I.Vetter, and A.Wittinghofer (1999).
Pictures in cell biology. Structures of nuclear-transport components.
  Trends Cell Biol, 9, 310-311.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB codes are shown on the right.

 

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