PDBsum entry 1h3p

Immunoglobulin

PDB id: 1h3p

Contents

Protein chains

215 a.a. *

220 a.a. *

Waters ×132

* Residue conservation analysis

PDB id:

1h3p

Name:

Immunoglobulin

Title:

Structural characterisation of a monoclonal antibody specific for the pres1 region of the hepatitis b virus

Structure:

Antibody fab fragment. Chain: h. Fragment: heavy chain. Antibody fab fragment. Chain: l. Fragment: light chain

Source:

Mus musculus. Mouse. Organism_taxid: 10090. Strain: balb/c. Cell_line: 5a19 hybridome. Cell: b-lymphocyte. Cell: b-lymphocyte

Biol. unit:

Dimer (from PDB file)

Resolution:

2.60Å R-factor: 0.213 R-free: 0.291

Authors:

J.C.Pizarro,B.Vulliez-Le-Normand,M.M.Riottot,A.Budkowska,G.A.Bentley

Key ref:

J.C.Pizarro et al. (2001). Structural and functional characterization of a monoclonal antibody specific for the preS1 region of hepatitis B virus. FEBS Lett, 509, 463-468. PubMed id: 11749974 DOI: 10.1016/S0014-5793(01)03190-8

Date:

12-Sep-02 Release date: 19-Sep-02

Supersedes:

1gm3

Protein chain

Q65ZL8  (Q65ZL8_MOUSE) -  VH7183-DSP2-JH3-CH1 protein (Fragment) from Mus musculus

Seq: 196 a.a.

Struc: 215 a.a.*

Protein chain

Q52L64  (Q52L64_MOUSE) -  ENSMUSG00000076577 protein from Mus musculus

Seq: 240 a.a.

Struc: 220 a.a.*

* PDB and UniProt seqs differ at 106 residue positions (black crosses)

DOI no: 10.1016/S0014-5793(01)03190-8

FEBS Lett 509:463-468 (2001)

PubMed id: 11749974

Structural and functional characterization of a monoclonal antibody specific for the preS1 region of hepatitis B virus.

J.C.Pizarro, B.Vulliez-le Normand, M.M.Riottot, A.Budkowska, G.A.Bentley.

ABSTRACT

The monoclonal antibody 5a19, raised against the ay serotype of hepatitis B virus, binds to the segment of the preS1 region comprising residues 37-43, which is implicated in attachment of the virus to hepatocytes. The dissociation constant, derived from kinetic studies using surface plasmon resonance techniques, is in the low nanomolar range. The nucleotide sequence of the variable domains has been determined and the corresponding germ-line genes have been identified. The three-dimensional structure of the Fab fragment has been determined by X-ray crystallography to 2.6 A resolution.

Selected figure(s)

Figure 1.
Fig. 1. Epitope mapping of 5a19 using the SPOTs test. The intensity of the spot indicates the avidity of the antibody. (a) Recognition of 6–15-mer peptides spanning the sequence 31–47 of the preS1 region (ay serotype). (b) The specificity of 5a19 was examined by substitution of each serotype-dependent residue by the one corresponding to the ad serotype (Gly35, Ser38, Asn39). See Section 3 for other mutations made at positions 36, 38 and 39.

Figure 3.
Fig. 3. A stereo view of the antigen-binding site of mAb 5a19, with V[H] on the left and V[L] on the right. Upper panel: surface representation with color coded for electrostatic potential, with red for negative charge and blue for positive charge. Lower panel: conformation of the antigen-binding loops, with CDR-1 in green, CDR-2 in mauve and CDR-3 in yellow. The figures were prepared with GRASP [21] and VMD [22], and both rendered with Raster3D [23].

The above figures are reprinted by permission from the Federation of European Biochemical Societies: FEBS Lett (2001, 509, 463-468) copyright 2001.

Figures were selected by an automated process.