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PDBsum entry 1gzc
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Sugar binding protein
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PDB id
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1gzc
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Contents |
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* Residue conservation analysis
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References listed in PDB file
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Key reference
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Title
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High-Resolution crystal structures of erythrina cristagalli lectin in complex with lactose and 2'-Alpha-L-Fucosyllactose and correlation with thermodynamic binding data.
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Authors
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C.Svensson,
S.Teneberg,
C.L.Nilsson,
A.Kjellberg,
F.P.Schwarz,
N.Sharon,
U.Krengel.
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Ref.
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J Mol Biol, 2002,
321,
69-83.
[DOI no: ]
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PubMed id
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Abstract
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The primary sequence of Erythrina cristagalli lectin (ECL) was mapped by mass
spectrometry, and the crystal structures of the lectin in complex with lactose
and 2'-alpha-L-fucosyllactose were determined at 1.6A and 1.7A resolution,
respectively. The two complexes were compared with the crystal structure of the
closely related Erythrina corallodendron lectin (ECorL) in complex with lactose,
with the crystal structure of the Ulex europaeus lectin II in complex with
2'-alpha-L-fucosyllactose, and with two modeled complexes of ECorL with
2'-alpha-L-fucosyl-N-acetyllactosamine. The molecular models are very similar to
the crystal structure of ECL in complex with 2'-alpha-L-fucosyllactose with
respect to the overall mode of binding, with the L-fucose fitting snugly into
the cavity surrounded by Tyr106, Tyr108, Trp135 and Pro134 adjoining the primary
combining site of the lectin. Marked differences were however noted between the
models and the experimental structure in the network of hydrogen bonds and
hydrophobic interactions holding the L-fucose in the combining site of the
lectin, pointing to limitations of the modeling approach. In addition to the
structural characterization of the ECL complexes, an effort was undertaken to
correlate the structural data with thermodynamic data obtained from
microcalorimetry, revealing the importance of the water network in the lectin
combining site for carbohydrate binding.
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Figure 3.
Figure 3. Electron density for lactose (a) and
fucosyllactose (b), in the respective ECL complexes. 2F[o] -F[c]
simulated-annealing omit maps covering the saccharides,
displayed at 1s.
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Figure 4.
Figure 4. Topology of ECL (complex with lactose). ECL
residues that differ from ECorL are shown explicity. In
addition, Val 92, the interaction partner of residues 111 and
125, is highlighted in orange.
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The above figures are
reprinted
by permission from Elsevier:
J Mol Biol
(2002,
321,
69-83)
copyright 2002.
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