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PDBsum entry 1gxr
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Transcription
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PDB id
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1gxr
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Contents |
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* Residue conservation analysis
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DOI no:
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Structure
10:751-761
(2002)
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PubMed id:
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Crystal structure of the C-terminal WD40 repeat domain of the human Groucho/TLE1 transcriptional corepressor.
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L.M.Pickles,
S.M.Roe,
E.J.Hemingway,
S.Stifani,
L.H.Pearl.
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ABSTRACT
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Groucho (Gro)/TLE proteins are transcriptional corepressors that lack inherent
DNA binding but interact with DNA-bound transcription factors and histones, and
recruit histone deacetylases. Groucho-mediated repression is essential in
embryonic development and involved in regulation of Wnt signaling in adult
tissue. We have determined the 1.6 A crystal structure of a C-terminal fragment
of human Groucho/TLE1, comprising part of the Ser/Pro-rich region and a
seven-bladed beta propeller WD40 repeat domain, implicated in protein-protein
interactions. The structure confirms the relationship to the yeast Tup1
corepressor, but reveals important structural differences specific to the
metazoan system. Analysis of missense mutations in the C. elegans Groucho
homolog UNC-37 identifies sites of interaction with repression effectors, and
suggests an induced fit binding site for eh1 domains of Engrailed-type
transcription factors.
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Selected figure(s)
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Figure 1.
Figure 1. Domain Structure of Groucho/TLE ProteinsSchematic
diagram of the defined domains in Gro/TLE corepressors.
Glutamine-rich Q domain; glycine/proline-rich GP domain; CcN
domain containing nuclear localization and phosphorylation
sites; serine/threonine-proline-rich SP domain; and WD40 repeat
domain. Residue numbers defining domain boundaries are for human
TLE1. Regions of Groucho/TLE proteins implicated in interaction
with transcription factors are indicated below (horizontal
lines), while those implicated in interactions with repression
effectors are indicated above. In many cases, involvement of a
particular region of Gro/TLE has only been defined by loss of
function on deletion of that region, and not by positive
demonstration of interaction.
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The above figure is
reprinted
by permission from Cell Press:
Structure
(2002,
10,
751-761)
copyright 2002.
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Figure was
selected
by an automated process.
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Literature references that cite this PDB file's key reference
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PubMed id
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Reference
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E.B.Flowers,
R.J.Poole,
B.Tursun,
E.Bashllari,
I.Pe'er,
and
O.Hobert
(2010).
The Groucho ortholog UNC-37 interacts with the short Groucho-like protein LSY-22 to control developmental decisions in C. elegans.
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Development,
137,
1799-1805.
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B.H.Jennings,
and
D.Ish-Horowicz
(2008).
The Groucho/TLE/Grg family of transcriptional co-repressors.
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Genome Biol,
9,
205.
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B.H.Jennings,
S.M.Wainwright,
and
D.Ish-Horowicz
(2008).
Differential in vivo requirements for oligomerization during Groucho-mediated repression.
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EMBO Rep,
9,
76-83.
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H.Ullah,
E.L.Scappini,
A.F.Moon,
L.V.Williams,
D.L.Armstrong,
and
L.C.Pedersen
(2008).
Structure of a signal transduction regulator, RACK1, from Arabidopsis thaliana.
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Protein Sci,
17,
1771-1780.
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PDB code:
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M.Buscarlet,
A.Perin,
A.Laing,
J.M.Brickman,
and
S.Stifani
(2008).
Inhibition of Cortical Neuron Differentiation by Groucho/TLE1 Requires Interaction with WRPW, but Not Eh1, Repressor Peptides.
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J Biol Chem,
283,
24881-24888.
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N.V.Valeyev,
A.K.Downing,
J.Sondek,
and
C.Deane
(2008).
Electrostatic and Functional Analysis of the Seven-Bladed WD beta-Propellers.
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Evol Bioinform Online,
4,
203-216.
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T.Suganuma,
S.G.Pattenden,
and
J.L.Workman
(2008).
Diverse functions of WD40 repeat proteins in histone recognition.
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Genes Dev,
22,
1265-1268.
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Z.Liu,
and
V.Karmarkar
(2008).
Groucho/Tup1 family co-repressors in plant development.
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Trends Plant Sci,
13,
137-144.
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F.Fagerström-Billai,
M.Durand-Dubief,
K.Ekwall,
and
A.P.Wright
(2007).
Individual subunits of the Ssn6-Tup11/12 corepressor are selectively required for repression of different target genes.
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Mol Cell Biol,
27,
1069-1082.
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H.Sun,
B.L.Nelms,
S.F.Sleiman,
H.M.Chamberlin,
and
W.Hanna-Rose
(2007).
Modulation of Caenorhabditis elegans transcription factor activity by HIM-8 and the related Zinc-Finger ZIM proteins.
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Genetics,
177,
1221-1226.
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M.Buscarlet,
and
S.Stifani
(2007).
The 'Marx' of Groucho on development and disease.
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Trends Cell Biol,
17,
353-361.
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B.H.Jennings,
L.M.Pickles,
S.M.Wainwright,
S.M.Roe,
L.H.Pearl,
and
D.Ish-Horowicz
(2006).
Molecular recognition of transcriptional repressor motifs by the WD domain of the Groucho/TLE corepressor.
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Mol Cell,
22,
645-655.
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PDB codes:
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B.Schlager,
W.Röseler,
M.Zheng,
A.Gutierrez,
and
R.J.Sommer
(2006).
HAIRY-like transcription factors and the evolution of the nematode vulva equivalence group.
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Curr Biol,
16,
1386-1394.
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C.W.Vander Kooi,
M.D.Ohi,
J.A.Rosenberg,
M.L.Oldham,
M.E.Newcomer,
K.L.Gould,
and
W.J.Chazin
(2006).
The Prp19 U-box crystal structure suggests a common dimeric architecture for a class of oligomeric E3 ubiquitin ligases.
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Biochemistry,
45,
121-130.
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PDB code:
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T.M.Malavé,
and
S.Y.Dent
(2006).
Transcriptional repression by Tup1-Ssn6.
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Biochem Cell Biol,
84,
437-443.
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D.K.Wilson,
D.Cerna,
and
E.Chew
(2005).
The 1.1-angstrom structure of the spindle checkpoint protein Bub3p reveals functional regions.
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J Biol Chem,
280,
13944-13951.
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PDB code:
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N.Marçal,
H.Patel,
Z.Dong,
S.Belanger-Jasmin,
B.Hoffman,
C.D.Helgason,
J.Dang,
and
S.Stifani
(2005).
Antagonistic effects of Grg6 and Groucho/TLE on the transcription repression activity of brain factor 1/FoxG1 and cortical neuron differentiation.
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Mol Cell Biol,
25,
10916-10929.
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S.R.Green,
and
A.D.Johnson
(2005).
Genome-wide analysis of the functions of a conserved surface on the corepressor Tup1.
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Mol Biol Cell,
16,
2605-2613.
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A.Y.Madrona,
and
D.K.Wilson
(2004).
The structure of Ski8p, a protein regulating mRNA degradation: Implications for WD protein structure.
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Protein Sci,
13,
1557-1565.
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PDB code:
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S.Znaidi,
B.Pelletier,
Y.Mukai,
and
S.Labbé
(2004).
The Schizosaccharomyces pombe corepressor Tup11 interacts with the iron-responsive transcription factor Fep1.
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J Biol Chem,
279,
9462-9474.
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T.E.Swingler,
K.L.Bess,
J.Yao,
S.Stifani,
and
P.S.Jayaraman
(2004).
The proline-rich homeodomain protein recruits members of the Groucho/Transducin-like enhancer of split protein family to co-repress transcription in hematopoietic cells.
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J Biol Chem,
279,
34938-34947.
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Z.Cheng,
Y.Liu,
C.Wang,
R.Parker,
and
H.Song
(2004).
Crystal structure of Ski8p, a WD-repeat protein with dual roles in mRNA metabolism and meiotic recombination.
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Protein Sci,
13,
2673-2684.
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PDB code:
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W.C.Voegtli,
A.Y.Madrona,
and
D.K.Wilson
(2003).
The structure of Aip1p, a WD repeat protein that regulates Cofilin-mediated actin depolymerization.
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J Biol Chem,
278,
34373-34379.
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PDB codes:
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The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
Where a reference describes a PDB structure, the PDB
code is
shown on the right.
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Links
