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PDBsum entry 1gdc
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Glucocorticoid receptor
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PDB id
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1gdc
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References listed in PDB file
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Key reference
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Title
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Refined solution structure of the glucocorticoid receptor DNA-Binding domain.
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Authors
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H.Baumann,
K.Paulsen,
H.Kovács,
H.Berglund,
A.P.Wright,
J.A.Gustafsson,
T.Härd.
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Ref.
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Biochemistry, 1993,
32,
13463-13471.
[DOI no: ]
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PubMed id
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Abstract
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A refined solution structure of the glucocorticoid receptor DNA-binding domain
(GR DBD) has been determined using two- and three-dimensional nuclear magnetic
resonance (NMR) spectroscopy on an 15N-labeled GR DBD fragment in conjunction
with distance geometry and simulated annealing calculations. Thirty structures
of the fragment C440-R510 of the rat GR were calculated based on 906 distance
constraints obtained from NOE intensities (168 intraresidue and 738 interresidue
NOEs) and 43 dihedral constraints. Average atomic root mean square (rms)
differences between the 24 best structures and their geometric average are 0.70
A for backbone atoms and 1.44 A for all heavy atoms. Several regions that were
not well defined in a previous NMR structure determination of a similar protein
fragment [Härd, T., Kellenbach, E., Boelens, R., Maler, B.A., Dahlman, K.,
Freedman, L.P., Carlstedt-Duke, J., Yamamoto, K.R., Gustafsson, J.-A., &
Kaptein, R. (1990b) Science 249, 157-160] are now well-defined. The refined
structure of the uncomplexed GR DBD is very similar to the crystal structure of
GR DBD in a sequence specific DNA complex [Luisi, B. F., Xu, W. X., Otwinowski,
Z., Freeman, L. P., Yamamoto, K. R., & Sigler, P. B. (1991) Nature 352,
497-505], in particular with regard to the presence and relative positions of
secondary structure elements. The backbone atom rms difference between the
average NMR solution structure and the crystal structure of the DNA-complexed GR
DBD is 1.8 A. The most pronounced differences between the free and DNA-complexed
states are found within the fragment C476-C482 in the second zinc-coordinating
domain.(ABSTRACT TRUNCATED AT 250 WORDS)
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Secondary reference #1
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Title
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Structure and function of the DNA-Binding domain of the glucocorticoid receptor and other members of the nuclear receptor supergene family
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Authors
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T.Hard,
J.-A.Gustafsson.
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Ref.
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acc chem res, 1993,
26,
644.
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Secondary reference #2
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Title
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Backbone dynamics of the glucocorticoid receptor DNA-Binding domain.
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Authors
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H.Berglund,
H.Kovács,
K.Dahlman-Wright,
J.A.Gustafsson,
T.Härd.
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Ref.
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Biochemistry, 1992,
31,
12001-12011.
[DOI no: ]
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PubMed id
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Secondary reference #3
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Title
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Crystallographic analysis of the interaction of the glucocorticoid receptor with DNA.
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Authors
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B.F.Luisi,
W.X.Xu,
Z.Otwinowski,
L.P.Freedman,
K.R.Yamamoto,
P.B.Sigler.
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Ref.
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Nature, 1991,
352,
497-505.
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PubMed id
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Secondary reference #4
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Title
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1h nmr studies of the glucocorticoid receptor DNA-Binding domain: sequential assignments and identification of secondary structure elements.
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Authors
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T.Härd,
E.Kellenbach,
R.Boelens,
R.Kaptein,
K.Dahlman,
J.Carlstedt-Duke,
L.P.Freedman,
B.A.Maler,
E.I.Hyde,
J.A.Gustafsson.
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Ref.
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Biochemistry, 1990,
29,
9015-9023.
[DOI no: ]
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PubMed id
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Secondary reference #5
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Title
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Solution structure of the glucocorticoid receptor DNA-Binding domain.
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Authors
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T.Härd,
E.Kellenbach,
R.Boelens,
B.A.Maler,
K.Dahlman,
L.P.Freedman,
J.Carlstedt-Duke,
K.R.Yamamoto,
J.A.Gustafsson,
R.Kaptein.
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Ref.
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Science, 1990,
249,
157-160.
[DOI no: ]
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PubMed id
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