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UniProt functional annotation for P0A6D3 | ||
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| UniProt codes: P0A6D3, P07638. |
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| Organism: | |
Escherichia coli (strain K12). |
| Taxonomy: | |
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia. |
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| Function: | |
Catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate (PEP) to the 5-hydroxyl of shikimate-3-phosphate (S3P) to produce enolpyruvyl shikimate-3-phosphate and inorganic phosphate. {ECO:0000255|HAMAP-Rule:MF_00210, ECO:0000269|PubMed:12430021, ECO:0000269|PubMed:13129913, ECO:0000269|PubMed:16225867, ECO:0000269|PubMed:17855366, ECO:0000269|PubMed:17958399, ECO:0000269|PubMed:19211556, ECO:0000269|PubMed:6229418}. |
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| Catalytic activity: | |
Reaction=3-phosphoshikimate + phosphoenolpyruvate = 5-O-(1- carboxyvinyl)-3-phosphoshikimate + phosphate; Xref=Rhea:RHEA:21256, ChEBI:CHEBI:43474, ChEBI:CHEBI:57701, ChEBI:CHEBI:58702, ChEBI:CHEBI:145989; EC=2.5.1.19; Evidence={ECO:0000255|HAMAP- Rule:MF_00210, ECO:0000269|PubMed:12430021, ECO:0000269|PubMed:13129913, ECO:0000269|PubMed:16225867, ECO:0000269|PubMed:17855366, ECO:0000269|PubMed:17958399, ECO:0000269|PubMed:19211556, ECO:0000269|PubMed:6229418}; |
| Activity regulation: | |
Competitively inhibited by glyphosate (PubMed:12430021, PubMed:6229418). Inhibited by (S)- and (R)- phosphonates analogs (PubMed:15736934). Inhibited by (R)-difluoromethyl analogs of the tetrahedral reaction intermediate (PubMed:16225867). Inhibited by bromopyruvate (PubMed:1899181). {ECO:0000269|PubMed:12430021, ECO:0000269|PubMed:15736934, ECO:0000269|PubMed:16225867, ECO:0000269|PubMed:17958399, ECO:0000269|PubMed:1899181, ECO:0000269|PubMed:6229418}. |
| Biophysicochemical properties: | |
Kinetic parameters: KM=0.0035 mM for S3P (at pH 7 and 25 degrees Celsius {ECO:0000269|PubMed:6229418}; KM=0.015 mM for PEP (at pH 7 and 25 degrees Celsius {ECO:0000269|PubMed:6229418}; KM=0.045 mM for PEP (at pH 7.5 and 25 degrees Celsius {ECO:0000269|PubMed:19211556}; KM=0.048 mM for S3P (at pH 7.5 and 25 degrees Celsius {ECO:0000269|PubMed:19211556}; KM=0.06 mM for PEP (at pH 7.5 and 25 degrees Celsius {ECO:0000269|PubMed:17855366}; KM=0.06 mM for S3P (at pH 7.5 and 25 degrees Celsius {ECO:0000269|PubMed:17855366}; KM=0.088 mM for PEP (at pH 6.8 and 20 degrees Celsius {ECO:0000269|PubMed:12430021}; KM=0.09 mM for S3P (at pH 5.5 and 20 degrees Celsius {ECO:0000269|PubMed:16225867}; KM=0.1 mM for PEP (at pH 5.5 and 20 degrees Celsius {ECO:0000269|PubMed:16225867}; KM=0.12 mM for S3P (at pH 6.8 and 20 degrees Celsius {ECO:0000269|PubMed:12430021}; Vmax=50 umol/min/ug enzyme (at pH 7.5 and 25 degrees Celsius {ECO:0000269|PubMed:17855366}; Vmax=53 umol/min/ug enzyme (at pH 5.5 and 20 degrees Celsius {ECO:0000269|PubMed:16225867}; Vmax=57 umol/min/ug enzyme (at pH 7.5 and 25 degrees Celsius {ECO:0000269|PubMed:19211556}; pH dependence: Optimum pH is between 6 and 6.5. {ECO:0000269|PubMed:12430021, ECO:0000269|PubMed:16225867, ECO:0000269|PubMed:17855366, ECO:0000269|PubMed:19211556, ECO:0000269|PubMed:6229418}; |
| Pathway: | |
Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7. {ECO:0000255|HAMAP-Rule:MF_00210, ECO:0000305}. |
| Subunit: | |
Monomer. {ECO:0000255|HAMAP-Rule:MF_00210, ECO:0000269|PubMed:6229418}. |
| Subcellular location: | |
Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00210, ECO:0000305}. |
| Similarity: | |
Belongs to the EPSP synthase family. {ECO:0000255|HAMAP- Rule:MF_00210, ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.