PDBsum entry 1g6j

Gene regulation, cell cycle

PDB id: 1g6j

Contents

Protein chain

76 a.a. *

* Residue conservation analysis

References listed in PDB file

Key reference

Title

Validation of protein structure from preparations of encapsulated proteins dissolved in low viscosity fluids.

Authors

C.R.Babu, P.F.Flynn, A.J.Wand.

Ref.

J Am Chem Soc, 2001, 123, 2691-2692. [DOI no: 10.1021/ja005766d]

PubMed id

11456950

Abstract

No abstract given.

Secondary reference #1

Title

High-Resolution nmr of encapsulated proteins dissolved in low-Viscosity fluids.

Authors

A.J.Wand, M.R.Ehrhardt, P.F.Flynn.

Ref.

Proc Natl Acad Sci U S A, 1998, 95, 15299-15302. [DOI no: 10.1073/pnas.95.26.15299]

PubMed id

9860963

Figure 1.
Fig. 1. 15N- heteronuclear single quantum correlation spectrum of 15N-labeled recombinant human ubiquitin encapsulated in AOT reverse micelles dissolved in liquid propane. The protein concentration was 0.3 mM. The spectrum was acquired at 750 MHz (1H) by using an 8-mm probe in 30 min at 25°C and 12 bar. One hundred and fifty complex points were collected in the incremented nitrogen time domain.

Figure 3.
Fig. 3. Reduction of amide 15N spin-spin relaxation rate in recombinant human ubiquitin encapsulated in a reverse micelle. Spin-spin relaxation rates (1/T[2]) are plotted against the bulk solvent viscosity (30, 31). Data points, in order of decreasing viscosity, are for iso-octane, n-heptane, n-hexane, n-pentane, n-butane, and propane. All data were collected at 25°C. The butane and propane samples were prepared at pressures of 7 and 12 bar, respectively. All other samples were prepared at ambient pressure.

Secondary reference #2

Title

Preparation of encapsulated proteins dissolved in low viscosity fluids.

Authors

M.R.Ehrhardt, P.F.Flynn, A.J.Wand.

Ref.

J Biomol Nmr, 1999, 14, 75-78.

PubMed id

10382308