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UniProt functional annotation for Q9YGA6 | ||
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| UniProt code: Q9YGA6. |
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| Organism: | |
Thermococcus litoralis (strain ATCC 51850 / DSM 5473 / JCM 8560 / NS-C). |
| Taxonomy: | |
Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae; Thermococcus. |
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| Function: | |
Part of the ABC transporter complex MalEFGK involved in trehalose/maltose import. Responsible for energy coupling to the transport system. {ECO:0000269|PubMed:10400644, ECO:0000269|PubMed:11453995, ECO:0000269|PubMed:8759837}. |
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| Catalytic activity: | |
Reaction=ATP + D-maltose(out) + H2O = ADP + D-maltose(in) + H(+) + phosphate; Xref=Rhea:RHEA:22132, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17306, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=7.5.2.1; |
| Activity regulation: | |
Inhibited by N-ethylmaleimide but not by vanadate. {ECO:0000269|PubMed:10400644}. |
| Biophysicochemical properties: | |
Kinetic parameters: KM=155 uM for ATP {ECO:0000269|PubMed:10400644}; KM=430 uM for GTP {ECO:0000269|PubMed:10400644}; KM=870 uM for CTP {ECO:0000269|PubMed:10400644}; Vmax=0.55 umol/min/mg enzyme with ATP as substrate {ECO:0000269|PubMed:10400644}; Vmax=0.45 umol/min/mg enzyme with GTP as substrate {ECO:0000269|PubMed:10400644}; Vmax=0.32 umol/min/mg enzyme with CTP as substrate {ECO:0000269|PubMed:10400644}; pH dependence: Optimum pH is 7.0. {ECO:0000269|PubMed:10400644}; Temperature dependence: Optimum temperature is 80 degrees Celsius. {ECO:0000269|PubMed:10400644}; |
| Subunit: | |
Homodimer. The complex is composed of two ATP-binding proteins (MalK), two transmembrane proteins (MalG and MalF) and a solute-binding protein (MalE). {ECO:0000269|PubMed:11080142, ECO:0000269|PubMed:11453995, ECO:0000269|PubMed:12454482}. |
| Subcellular location: | |
Cell membrane {ECO:0000269|PubMed:11453995}; Peripheral membrane protein {ECO:0000269|PubMed:11453995}. |
| Induction: | |
Induced by maltose and trehalose. {ECO:0000269|PubMed:10400644}. |
| Domain: | |
Contains an N-terminal ATPase domain and a C-terminal regulatory domain. {ECO:0000269|PubMed:11080142}. |
| Similarity: | |
Belongs to the ABC transporter superfamily. Maltose/trehalose importer (TC 3.A.1.1.7) family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.