UniProt functional annotation for P14870

UniProt code: P14870.

Organism: Planomicrobium okeanokoites (Planococcus okeanokoites) (Flavobacterium okeanokoites).
Taxonomy: Bacteria; Firmicutes; Bacilli; Bacillales; Planococcaceae; Planomicrobium.
Function: Recognizes the double-stranded sequence 5'-GGATG-3'/3'- CATCC-5' and cleaves respectively 14 bases after G-1 and 13 bases before C-1.
Catalytic activity: Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.
Cofactor: Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
Subunit: Monomer, in which form it can cleave DNA. Homodimer when bound to DNA. {ECO:0000269|PubMed:9214510, ECO:0000269|PubMed:9724744}.
Domain: Has an N-terminal DNA recognition domain (approximately residues 1-292) joined to an endonuclease domain (residues 389- 583) by a flexible linker. {ECO:0000303|PubMed:9214510}.
Biotechnology: Adding the endonuclease domain C-terminal to different DNA recognition domains from other enzymes allows generation of novel restriction enzymes that are used to genetically engineer eukaryotic cells. {ECO:0000269|PubMed:24792163, ECO:0000303|PubMed:25057889}.
Sequence caution: Sequence=AAA24934.1; Type=Erroneous initiation; Note=Translation N-terminally extended; Evidence={ECO:0000305};

Annotations taken from UniProtKB at the EBI.