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PDBsum entry 1flr
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Immunoglobulin
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PDB id
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1flr
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Contents |
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* Residue conservation analysis
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References listed in PDB file
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Key reference
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Title
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1.85 a structure of anti-Fluorescein 4-4-20 FAB.
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Authors
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M.Whitlow,
A.J.Howard,
J.F.Wood,
E.W.Voss,
K.D.Hardman.
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Ref.
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Protein Eng, 1995,
8,
749-761.
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PubMed id
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Note In the PDB file this reference is
annotated as "TO BE PUBLISHED".
The citation details given above were identified by an automated
search of PubMed on title and author
names, giving a
percentage match of
85%.
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Abstract
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The crystal complex of fluorescein bound to the high-affinity anti-fluorescein
4-4-20 Fab (Ka = 10(10) M-1 at 2 degrees C) has been determined at 1.85 A.
Isomorphous crystals of two isoelectric forms (pI = 7.5 and 7.9) of the
anti-fluorescein 4-4-20 Fab, an IgG2A [Gibson et al. (1988) Proteins: Struct.
Funct. Genet., 3, 155-160], have been grown. Both complexes crystallize with one
molecule in the asymmetric unit in space group P1, with a = 42.75 A, b = 43.87
A, c = 58.17 A, alpha = 95.15 degrees, beta = 86.85 degrees and gamma = 98.01
degrees. The final structure has an R value of 0.188 at 1.85 A resolution.
Interactions between bound fluorescein, the complementarity-determining regions
(CDRs) of the Fab and the active-site mutants of the 4-4-20 single-chain Fv will
be discussed. Differences were found between the structure reported here and the
previously reported 2.7 A 4-4-20 Fab structure [Herron et al. (1989) Proteins:
Struct. Funct. Genet., 5, 271-280]. Our structure determination was based on
26,328 unique reflections--four times the amount of data used in the previous
report. Differences in the two structures could be explained by differences in
interpreting the electron density maps at the various resolutions. The r.m.s.
deviations between the variable and constant domains of the two structures were
0.77 and 1.54 A, respectively. Four regions of the light chain and four regions
of the heavy chain had r.m.s. backbone deviations of > 4 A. The most
significant of these was the conformation of the light chain CDR 1.
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Secondary reference #1
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Title
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Three-Dimensional structure of a fluorescein-Fab complex crystallized in 2-Methyl-2,4-Pentanediol.
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Authors
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J.N.Herron,
X.M.He,
M.L.Mason,
E.W.Voss,
A.B.Edmundson.
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Ref.
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Proteins, 1989,
5,
271-280.
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PubMed id
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Secondary reference #2
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Title
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Differences in crystal properties and ligand affinities of an antifluorescyl FAB (4-4-20) in two solvent systems.
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Authors
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A.L.Gibson,
J.N.Herron,
X.M.He,
V.A.Patrick,
M.L.Mason,
J.N.Lin,
D.M.Kranz,
E.W.Voss,
A.B.Edmundson.
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Ref.
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Proteins, 1988,
3,
155-160.
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PubMed id
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Secondary reference #3
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Title
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Comparison of variable region primary structures within an anti-Fluorescein idiotype family.
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Authors
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W.D.Bedzyk,
L.S.Johnson,
G.S.Riordan,
E.W.Voss.
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Ref.
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J Biol Chem, 1989,
264,
1565-1569.
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PubMed id
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