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214 a.a.
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221 a.a.
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129 a.a.
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* Residue conservation analysis
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PDB id:
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Complex (antibody/antigen)
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Title:
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Crystal structure of a cross-reaction complex between fab f9.13.7 and guinea-fowl lysozyme
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Structure:
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Igg1 f9.13.7 fab (light chain). Chain: l, p. Engineered: yes. Igg1 f9.13.7 fab (heavy chain). Chain: h, q. Engineered: yes. Guinea fowl lysozyme. Chain: x, y. Engineered: yes
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Source:
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Mus musculus. House mouse. Organism_taxid: 10090. Numida meleagris. Helmeted guineafowl. Organism_taxid: 8996
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Biol. unit:
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Trimer (from
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Resolution:
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Authors:
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J.Lescar,P.M.Alzari
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Key ref:
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J.Lescar
et al.
(1995).
Crystal structure of a cross-reaction complex between Fab F9.13.7 and guinea fowl lysozyme.
J Biol Chem,
270,
18067-18076.
PubMed id:
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Date:
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19-Jan-95
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Release date:
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27-Feb-95
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PROCHECK
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Headers
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References
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P01837
(IGKC_MOUSE) -
Immunoglobulin kappa constant from Mus musculus
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Seq:
Struc:
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107 a.a.
214 a.a.
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Enzyme class:
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Chains X, Y:
E.C.3.2.1.17
- lysozyme.
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Reaction:
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Hydrolysis of the 1,4-beta-linkages between N-acetyl-D-glucosamine and N-acetylmuramic acid in peptidoglycan heteropolymers of the prokaryotes cell walls.
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J Biol Chem
270:18067-18076
(1995)
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PubMed id:
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Crystal structure of a cross-reaction complex between Fab F9.13.7 and guinea fowl lysozyme.
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J.Lescar,
M.Pellegrini,
H.Souchon,
D.Tello,
R.J.Poljak,
N.Peterson,
M.Greene,
P.M.Alzari.
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ABSTRACT
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The crystal structure of the complex between the cross-reacting antigen Guinea
fowl lysozyme and the Fab from monoclonal antibody F9.13.7, raised against hen
egg lysozyme, has been determined by x-ray diffraction to 3-A resolution. The
antibody interacts with exposed residues of an alpha-helix and surrounding loops
adjacent to the lysozyme active site cleft. The epitope of lysozyme bound by
antibody F9.13.7 overlaps almost completely with that bound by antibody HyHEL10;
the same 12 residues of the antigen interact with the two antibodies. The
antibodies, however, have different combining sites with no sequence homology at
any of their complementarity-determining regions and show a dissimilar pattern
of cross-reactivity with heterologous antigens. Side chain mobility of epitope
residues contributes to confer steric and electrostatic complementarity to
differently shaped combining sites, allowing functional mimicry to occur. The
capacity of two antibodies that have different fine specificities to bind the
same area of the antigen emphasizes the operational character of the definition
of an antigenic determinant. This example demonstrates that degenerate binding
of the same structural motif does not require the existence of sequence homology
or other chemical similarities between the different binding sites.
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Literature references that cite this PDB file's key reference
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PubMed id
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Reference
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R.Rajamanonmani,
C.Nkenfou,
P.Clancy,
Y.H.Yau,
S.G.Shochat,
S.Sukupolvi-Petty,
W.Schul,
M.S.Diamond,
S.G.Vasudevan,
and
J.Lescar
(2009).
On a mouse monoclonal antibody that neutralizes all four dengue virus serotypes.
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J Gen Virol,
90,
799-809.
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M.Mo,
H.C.Lee,
M.Kotaka,
M.Niang,
X.Gao,
J.K.Iyer,
J.Lescar,
and
P.Preiser
(2008).
The C-terminal segment of the cysteine-rich interdomain of Plasmodium falciparum erythrocyte membrane protein 1 determines CD36 binding and elicits antibodies that inhibit adhesion of parasite-infected erythrocytes.
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Infect Immun,
76,
1837-1847.
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V.Moreau,
C.Fleury,
D.Piquer,
C.Nguyen,
N.Novali,
S.Villard,
D.Laune,
C.Granier,
and
F.Molina
(2008).
PEPOP: computational design of immunogenic peptides.
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BMC Bioinformatics,
9,
71.
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D.Paus,
and
G.Winter
(2006).
Mapping epitopes and antigenicity by site-directed masking.
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Proc Natl Acad Sci U S A,
103,
9172-9177.
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R.J.Duquesnoy
(2006).
A structurally based approach to determine HLA compatibility at the humoral immune level.
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Hum Immunol,
67,
847-862.
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A.Cauerhff,
F.A.Goldbaum,
and
B.C.Braden
(2004).
Structural mechanism for affinity maturation of an anti-lysozyme antibody.
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Proc Natl Acad Sci U S A,
101,
3539-3544.
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PDB code:
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G.Johnson,
and
S.W.Moore
(2004).
Functional idiotypic mimicry of an adhesion- and differentiation-promoting site on acetylcholinesterase.
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J Cell Biochem,
91,
999.
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S.Mohan,
N.Sinha,
and
S.J.Smith-Gill
(2003).
Modeling the binding sites of anti-hen egg white lysozyme antibodies HyHEL-8 and HyHEL-26: an insight into the molecular basis of antibody cross-reactivity and specificity.
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Biophys J,
85,
3221-3236.
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H.Oliva,
B.Moltedo,
P.De Ioannes,
F.Faunes,
A.E.De Ioannes,
and
M.I.Becker
(2002).
Monoclonal antibodies to molluskan hemocyanin from Concholepas concholepas demonstrate common and specific epitopes among subunits.
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Hybrid Hybridomics,
21,
365-374.
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J.Pons,
J.R.Stratton,
and
J.F.Kirsch
(2002).
How do two unrelated antibodies, HyHEL-10 and F9.13.7, recognize the same epitope of hen egg-white lysozyme?
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Protein Sci,
11,
2308-2315.
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G.Cunto-Amesty,
P.Luo,
B.Monzavi-Karbassi,
and
T.Kieber-Emmons
(2001).
Exploiting molecular mimicry: defining rules of the game.
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Int Rev Immunol,
20,
157-180.
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D.W.Ritchie,
and
G.J.Kemp
(2000).
Protein docking using spherical polar Fourier correlations.
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Proteins,
39,
178-194.
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P.B.Furtado,
R.Furmonaviciene,
J.McElveen,
H.F.Sewell,
and
F.Shakib
(2000).
Prediction of the interacting surfaces in a trimolecular complex formed between the major dust mite allergen Der p 1, a mouse monoclonal anti-Der p 1 antibody, and its anti-idiotype.
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Mol Pathol,
53,
324-332.
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J.M.van Den Elsen,
D.A.Kuntz,
F.J.Hoedemaeker,
and
D.R.Rose
(1999).
Antibody C219 recognizes an alpha-helical epitope on P-glycoprotein.
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Proc Natl Acad Sci U S A,
96,
13679-13684.
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PDB code:
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M.H.Seto,
H.L.Liu,
D.A.Zajchowski,
and
M.Whitlow
(1999).
Protein fold analysis of the B30.2-like domain.
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Proteins,
35,
235-249.
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C.Demangel,
S.Rouyre,
P.M.Alzari,
F.Nato,
S.Longacre,
P.Lafaye,
and
J.C.Mazie
(1998).
Phage-displayed mimotopes elicit monoclonal antibodies specific for a malaria vaccine candidate.
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Biol Chem,
379,
65-70.
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J.P.Bouvet,
and
G.Dighiero
(1998).
From natural polyreactive autoantibodies to à la carte monoreactive antibodies to infectious agents: is it a small world after all?
|
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Infect Immun,
66,
1-4.
|
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G.Dighiero
(1997).
Natural autoantibodies, tolerance, and autoimmunity.
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Ann N Y Acad Sci,
815,
182-192.
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I.A.Wilson,
and
K.C.Garcia
(1997).
T-cell receptor structure and TCR complexes.
|
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Curr Opin Struct Biol,
7,
839-848.
|
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|
|
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A.Desmyter,
T.R.Transue,
M.A.Ghahroudi,
M.H.Thi,
F.Poortmans,
R.Hamers,
S.Muyldermans,
and
L.Wyns
(1996).
Crystal structure of a camel single-domain VH antibody fragment in complex with lysozyme.
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Nat Struct Biol,
3,
803-811.
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PDB code:
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B.A.Fields,
F.A.Goldbaum,
W.Dall'Acqua,
E.L.Malchiodi,
A.Cauerhff,
F.P.Schwarz,
X.Ysern,
R.J.Poljak,
and
R.A.Mariuzza
(1996).
Hydrogen bonding and solvent structure in an antigen-antibody interface. Crystal structures and thermodynamic characterization of three Fv mutants complexed with lysozyme.
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Biochemistry,
35,
15494-15503.
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PDB codes:
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The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
Where a reference describes a PDB structure, the PDB
code is
shown on the right.
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Links
