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PDBsum entry 1f5y
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Lipid binding protein
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PDB id
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1f5y
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Contents |
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* Residue conservation analysis
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References listed in PDB file
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Key reference
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Title
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Nmr structure of a concatemer of the first and second ligand-Binding modules of the human low-Density lipoprotein receptor.
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Authors
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N.D.Kurniawan,
A.R.Atkins,
S.Bieri,
C.J.Brown,
I.M.Brereton,
P.A.Kroon,
R.Smith.
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Ref.
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Protein Sci, 2000,
9,
1282-1293.
[DOI no: ]
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PubMed id
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Abstract
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The ligand-binding domain of the human low-density lipoprotein receptor consists
of seven modules, each of 40-45 residues. In the presence of calcium, these
modules adopt a common polypeptide fold with three conserved disulfide bonds. A
concatemer of the first and second modules (LB(1-2)) folds efficiently in the
presence of calcium ions, forming the same disulfide connectivities as in the
isolated modules. The three-dimensional structure of LB(1-2) has now been solved
using two-dimensional 1H NMR spectroscopy and restrained molecular dynamics
calculations. No intermodule nuclear Overhauser effects were observed,
indicating the absence of persistent interaction between them. The near
random-coil NH and H alpha chemical shifts and the low phi and psi angle order
parameters of the four-residue linker suggest that it has considerable
flexibility. The family of LB(1-2) structures superimposed well over LB1 or LB2,
but not over both modules simultaneously. LB1 and LB2 have a similar pattern of
calcium ligands, but the orientations of the indole rings of the tryptophan
residues W23 and W66 differ, with the latter limiting solvent access to the
calcium ion. From these studies, it appears that although most of the modules in
the ligand-binding region of the receptor are joined by short segments, these
linkers may impart considerable flexibility on this region.
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Secondary reference #1
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Title
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Calcium is essential for the structural integrity of the cysteine-Rich, Ligand-Binding repeat of the low-Density lipoprotein receptor.
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Authors
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A.R.Atkins,
I.M.Brereton,
P.A.Kroon,
H.T.Lee,
R.Smith.
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Ref.
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Biochemistry, 1998,
37,
1662-1670.
[DOI no: ]
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PubMed id
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Secondary reference #2
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Title
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Folding, Calcium binding, And structural characterization of a concatemer of the first and second ligand-Binding modules of the low-Density lipoprotein receptor.
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Authors
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S.Bieri,
A.R.Atkins,
H.T.Lee,
D.J.Winzor,
R.Smith,
P.A.Kroon.
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Ref.
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Biochemistry, 1998,
37,
10994-11002.
[DOI no: ]
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PubMed id
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Secondary reference #3
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Title
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Three-Dimensional structure of a cysteine-Rich repeat from the low-Density lipoprotein receptor.
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Authors
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N.L.Daly,
M.J.Scanlon,
J.T.Djordjevic,
P.A.Kroon,
R.Smith.
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Ref.
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Proc Natl Acad Sci U S A, 1995,
92,
6334-6338.
[DOI no: ]
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PubMed id
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Secondary reference #4
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Title
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Three-Dimensional structure of the second cysteine-Rich repeat from the human low-Density lipoprotein receptor.
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Authors
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N.L.Daly,
J.T.Djordjevic,
P.A.Kroon,
R.Smith.
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Ref.
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Biochemistry, 1995,
34,
14474-14481.
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PubMed id
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