PDBsum entry 1f2s

Hydrolase/hydrolase inhibitor

PDB id: 1f2s

Contents

Protein chains

223 a.a. *

28 a.a. *

Metals

_CA

Waters ×207

* Residue conservation analysis

References listed in PDB file

Key reference

• Title: Crystal structure of the complex formed between bovine beta-Trypsin and mcti-A, A trypsin inhibitor of squash family, At 1.8-A resolution.
• Authors: Y.Zhu, Q.Huang, M.Qian, Y.Jia, Y.Tang.
• Ref.: J Protein Chem, 1999, 18, 505-509.
• PubMed id: 10524768

Abstract

The stoichiometric complex formed between bovine beta-trypsin and Momordica charantia, Linn. Cucurbitaceae trypsin inhibitor A (MCTI-A) was crystallized and its X-ray crystal structure was refined to a final R value of 0.179 using data of 7.0- to 1.8-A resolution. Combination with results on the complex of MCTI-A with porcine trypsin gives the sequence of MCTI-A definitely, of which 13 residues are conserved compared with other squash family trypsin inhibitors. Its spatial structure and the conformation of its primary binding segment from Cys3I (P3) to Glu7I (P3'), which contains a reactive scissile bond Arg5I C-Ile6I N, were found to be very similar to the other squash family proteinase inhibitors.

Secondary reference #1

• Title: Refined 1.6 a resolution crystal structure of the complex formed between porcine beta-Trypsin and mcti-A, A trypsin inhibitor of the squash family. Detailed comparison with bovine beta-Trypsin and its complex.
• Authors: Q.Huang, S.Liu, Y.Tang.
• Ref.: J Mol Biol, 1993, 229, 1022-1036.
• PubMed id: 8445634

Secondary reference #2

• Title: Amino acid sequencing of a trypsin inhibitor by refined 1.6 a X-Ray crystal structure of its complex with porcine beta-Trypsin.
• Authors: Q.Huang, S.Liu, Y.Tang, F.Zeng, R.Qian.
• Ref.: FEBS Lett, 1992, 297, 143-146. [DOI no: 10.1016/0014-5793(92)80346-I]
• PubMed id: 1551419