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UniProt functional annotation for P0A1P6 | ||
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| UniProt code: P0A1P6. |
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| Organism: | |
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720). |
| Taxonomy: | |
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Salmonella. |
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| Function: | |
Catalyzes the ATP-dependent biosynthesis of glutamine from glutamate and ammonia. {ECO:0000269|PubMed:7727369}. |
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| Catalytic activity: | |
Reaction=ATP + L-glutamate + NH4(+) = ADP + H(+) + L-glutamine + phosphate; Xref=Rhea:RHEA:16169, ChEBI:CHEBI:15378, ChEBI:CHEBI:28938, ChEBI:CHEBI:29985, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:58359, ChEBI:CHEBI:456216; EC=6.3.1.2; Evidence={ECO:0000305|PubMed:7727369}; |
| Cofactor: | |
Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000269|PubMed:11329256, ECO:0000269|PubMed:2572586, ECO:0000269|PubMed:7727369, ECO:0000269|PubMed:8099447}; Note=Binds 2 Mn(2+) ions per subunit. {ECO:0000269|PubMed:11329256, ECO:0000269|PubMed:2572586, ECO:0000269|PubMed:7727369, ECO:0000269|PubMed:8099447}; |
| Activity regulation: | |
When cellular nitrogen levels are high, the C- terminal adenylyl transferase (AT) of GlnE inhibits GlnA by covalent transfer of an adenylyl group from ATP to Tyr-398. Conversely, when nitrogen levels are low, the N-terminal adenylyl removase (AR) of GlnE activates GlnA by removing the adenylyl group by phosphorolysis. The fully adenylated enzyme complex is inactive. {ECO:0000250|UniProtKB:Q3V5W6}. |
| Biophysicochemical properties: | |
Kinetic parameters: KM=0.58 mM for ATP {ECO:0000269|PubMed:7727369}; |
| Subunit: | |
Oligomer of 12 subunits arranged in the form of two hexameric ring. {ECO:0000269|PubMed:11329256, ECO:0000269|PubMed:2572586, ECO:0000269|PubMed:7727369, ECO:0000269|PubMed:8099447}. |
| Subcellular location: | |
Cytoplasm {ECO:0000250|UniProtKB:P9WN39}. |
| Similarity: | |
Belongs to the glutamine synthetase family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.