PDBsum entry 1f13

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Coagulation factor PDB id
Protein chain
722 a.a. *
Waters ×487
* Residue conservation analysis

References listed in PDB file
Key reference
Title Two non-Proline cis peptide bonds may be important for factor xiii function.
Authors M.S.Weiss, H.J.Metzner, R.Hilgenfeld.
Ref. FEBS Lett, 1998, 423, 291-296. [DOI no: 10.1016/S0014-5793(98)00098-2]
PubMed id 9515726
The structure of recombinant human cellular factor XIII zymogen was solved in its monoclinic crystal form and refined to an R-factor of 18.3% (Rfree = 23.6%) for all data between 40.0 and 2.1 A resolution. Two non-proline cis peptide bonds were detected. One is between Arg310 and Tyr311 close to the active site cysteine residue (Cys314) and the other is between Gln425 and Phe426 at the dimerization interface. The structure and the role of these cis peptides are discussed in the light of their possible importance for factor XIII function.
Figure 1.
Fig. 1. Overall structure of the factor XIII dimer. The position of the local twofold axis is indicated by (). Within one subunit, the four domains are coloured in yellow, red, green and blue, respectively. The sites of the cis peptide bonds are emphasized by *. The figure was produced using the program MOLSCRIPT [28].
Figure 4.
Fig. 4. Sequence alignment of human factor XIII with representative transglutaminases in the regions of the cis peptide bonds. The code given is the SWISSPROT database code, and the identity is with respect to the alignment against factor XIII. The number of amino acids denotes the total length of the aligned region. The residues directly flanking the cis peptide bonds are inside the boxes.
The above figures are reprinted by permission from the Federation of European Biochemical Societies: FEBS Lett (1998, 423, 291-296) copyright 1998.
Secondary reference #1
Title Structural evidence that the activation peptide is not released upon thrombin cleavage of factor xiii.
Authors V.C.Yee, L.C.Pedersen, P.D.Bishop, R.E.Stenkamp, D.C.Teller.
Ref. Thromb Res, 1995, 78, 389-397.
PubMed id 7660355
Secondary reference #2
Title Three-Dimensional structure of a transglutaminase: human blood coagulation factor xiii.
Authors V.C.Yee, L.C.Pedersen, I.Le trong, P.D.Bishop, R.E.Stenkamp, D.C.Teller.
Ref. Proc Natl Acad Sci U S A, 1994, 91, 7296-7300. [DOI no: 10.1073/pnas.91.15.7296]
PubMed id 7913750
Full text Abstract
Secondary reference #3
Title Crystallization of blood coagulation factor xiii by an automated procedure.
Authors R.Hilgenfeld, A.Liesum, R.Storm, H.J.Metzner, H.E.Karges.
Ref. Febs Lett, 1990, 265, 110-112.
PubMed id 2365049
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