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PDBsum entry 1epj
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Growth factor
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PDB id
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1epj
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References listed in PDB file
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Key reference
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Title
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Three-Dimensional nuclear magnetic resonance structures of mouse epidermal growth factor in acidic and physiological ph solutions.
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Authors
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D.Kohda,
F.Inagaki.
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Ref.
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Biochemistry, 1992,
31,
11928-11939.
[DOI no: ]
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PubMed id
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Abstract
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The three-dimensional structures of epidermal growth factors (EGF) previously
reported were all in acidic solutions (pH 2.0-3.2), at which pHs EGF cannot bind
to the receptor. Here we studied the structure of mouse EGF at pH 6.8, where EGF
is physiologically active, and compared it with the structure at pH 2.0 by CD
and NMR. From pH dependence of CD spectra and a comparison between the chemical
shifts of the proton resonances at pH 6.8 and 2.0, the conformations at two pHs
were found to be nearly identical except for the C-terminal tail region. The
three-dimensional structures at pH 6.8 and 2.0 were determined independently by
a combination of two-dimensional 1H NMR and stimulated annealing calculations
using the program XPLOR. The calculations were based on 261 distance constraints
at pH 6.8 and 355 distance and 24 torsion angle constraints at pH 2.0. The
conformational difference of the C-terminal domain (residues 33-50) was detected
between the two structures, which were supported by CD and the chemical shift
comparison. The positions of the side chains of Leu47, Arg48, Trp49, and Trp50
are changed probably by the effect of the deprotonation of Asp46. Considering
the fact that Leu47 is essential in EGF binding to the receptor, this
conformational difference may be important in receptor recognition.
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Secondary reference #1
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Title
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Structure of epidermal growth factor bound to perdeuterated dodecylphosphocholine micelles determined by two-Dimensional nmr and simulated annealing calculations.
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Authors
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D.Kohda,
F.Inagaki.
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Ref.
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Biochemistry, 1992,
31,
677-685.
[DOI no: ]
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PubMed id
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Secondary reference #2
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Title
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Characterization of ph titration shifts for all the nonlabile proton resonances a protein by two-Dimensional nmr: the case of mouse epidermal growth factor.
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Authors
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D.Kohda,
T.Sawada,
F.Inagaki.
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Ref.
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Biochemistry, 1991,
30,
4896-4900.
[DOI no: ]
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PubMed id
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Secondary reference #3
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Title
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Tertiary structure of mouse epidermal growth factor determined by two-Dimensional 1h nmr.
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Authors
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D.Kohda,
N.Go,
K.Hayashi,
F.Inagaki.
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Ref.
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J Biochem (tokyo), 1988,
103,
741-743.
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PubMed id
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Secondary reference #4
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Title
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A comparative 1h nmr study of mouse alpha(1-53) and beta(2-53) epidermal growth factors.
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Authors
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D.Kohda,
C.Kodama,
R.Kase,
H.Nomoto,
K.Hayashi,
F.Inagaki.
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Ref.
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Biochem Int, 1988,
16,
647-654.
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PubMed id
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Secondary reference #5
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Title
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Complete sequence-Specific 1h nuclear magnetic resonance assignments for mouse epidermal growth factor.
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Authors
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D.Kohda,
F.Inagaki.
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Ref.
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J Biochem (tokyo), 1988,
103,
554-571.
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PubMed id
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